| Title |
Generation of new compounds through unbalanced transcription of landomycin A cluster
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, July 2016
|
| DOI | 10.1007/s00253-016-7721-3 |
| Pubmed ID | |
| Authors |
Maksym Myronovskyi, Elke Brötz, Birgit Rosenkränzer, Niko Manderscheid, Bogdan Tokovenko, Yuriy Rebets, Andriy Luzhetskyy |
| Abstract |
The biosynthetically well-studied landomycin A cluster has been used to demonstrate the unbalancing of gene transcription as an efficient method for the generation of new compounds. Landomycin A structural genes were decoupled from the native regulators LanI and LanK and placed under the control of a single synthetic promoter and expressed in a heterologous host Streptomyces albus J1074. In contrast to their native quantitative and temporal regulation, these genes were transcribed as a single polycistronic mRNA leading to the production of four novel and two known compounds. No glycosylated landomycins were detected though the entire biosynthetic cluster was transcribed, showing the crucial role of the balanced gene expression for the production of landomycin A. Two new compounds, fridamycin F and G, isolated in this study were shown to originate from the interplay between the expressed biosynthetic pathway and metabolic network of the heterologous host. Structure activity studies of the isolated compounds as well as results of transcriptome sequencing are discussed in this article. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 35 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 8 | 23% |
| Researcher | 6 | 17% |
| Student > Ph. D. Student | 4 | 11% |
| Student > Bachelor | 2 | 6% |
| Professor > Associate Professor | 2 | 6% |
| Other | 2 | 6% |
| Unknown | 11 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 12 | 34% |
| Agricultural and Biological Sciences | 8 | 23% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
| Unspecified | 1 | 3% |
| Immunology and Microbiology | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 12 | 34% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 July 2016.
All research outputs
#16,371,088
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#5,817
of 8,034 outputs
Outputs of similar age
#233,638
of 361,063 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#57
of 111 outputs
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