| Title |
Histone-dependent IgG conservation in octanoic acid precipitation and its mechanism
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, July 2016
|
| DOI | 10.1007/s00253-016-7719-x |
| Pubmed ID | |
| Authors |
Quan Chen, Phyllicia Toh, Yue Sun, Sarah Maria Abdul Latiff, Aina Hoi, Mo Xian, Haibo Zhang, Rui Nian, Wei Zhang, Pete Gagnon |
| Abstract |
Octanoic acid (OA) precipitation has long been used in protein purification. Recently, we reported a new cell culture clarification method for immunoglobulin G (IgG) purification, employing an advance elimination of chromatin heteroaggregates with a hybrid OA-solid phase system. This treatment reduced DNA more than 3 logs, histone below the detection limit (LOD), and non-histone host cell proteins (nh-HCP) by 90 % while conserving more than 90 % of the IgG monomer. In this study, we further investigated the conservation of IgG monomer and antibody light chain (LC) to the addition of OA/OA-solid phase complex, with or without histone and DNA in different combinations. The results showed that highly basic histone protein was the prime target in OA/OA-solid phase precipitation system for IgG purification, and the selective conservation of IgG monomer in this system was histone dependent. Our findings partially support the idea that OA works by sticking to electropositive hydrophobic domains on proteins, reducing their solubility, and causing them to agglomerate into large particles that precipitate from solution. Our findings also provide a new perspective for IgG purification and emphasize the necessity to re-examine the roles of various host contaminants in IgG purification. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Sweden | 1 | 4% |
| Unknown | 27 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 9 | 32% |
| Student > Ph. D. Student | 4 | 14% |
| Student > Bachelor | 4 | 14% |
| Student > Doctoral Student | 2 | 7% |
| Student > Master | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 7 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 9 | 32% |
| Agricultural and Biological Sciences | 4 | 14% |
| Chemical Engineering | 2 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
| Nursing and Health Professions | 1 | 4% |
| Other | 3 | 11% |
| Unknown | 8 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 May 2022.
All research outputs
#4,986,314
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#1,213
of 8,034 outputs
Outputs of similar age
#85,882
of 361,551 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#15
of 110 outputs
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So far Altmetric has tracked 8,034 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done well, scoring higher than 84% of its peers.
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We're also able to compare this research output to 110 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 86% of its contemporaries.