| Title |
Species Dependence for Binding of Small Molecule Agonist and Antagonists to the C5a Receptor on Polymorphonuclear Leukocytes
|
|---|---|
| Published in |
Inflammation, June 2001
|
| DOI | 10.1023/a:1011036414353 |
| Pubmed ID | |
| Authors |
Trent M. Woodruff, Anna J. Strachan, Sam D. Sanderson, Peter N. Monk, Allan K. Wong, David P. Fairlie, Stephen M. Taylor |
| Abstract |
This study investigated the receptor binding affinities of a C5a agonist and cyclic antagonists for polymorphonuclear leukocytes (PMNs) isolated from human, sheep, pig, dog, rabbit, guinea pig, rat and mouse. The affinities of the two small molecule antagonists, F-[OPdChaWR] and AcF-[OPdChaWR], and the agonist, YSFKPMPLaR, revealed large differences in C5a receptor (C5aR) affinities between species. The antagonists bound to human, rat and dog PMNs with similar high affinities, but with lower affinities to PMNs from all other species. The C5a agonist also bound with varying affinities between species, but showed a different affinity profile to the antagonists. In contrast, recombinant human C5a had similar affinity for PMNs of all species investigated. The low correlation between the affinities of the antagonists and the agonist between species either suggests that different receptor residues are important for distinguishing between agonist/antagonist binding, or that the agonist and antagonist peptides bind to two distinct sites within the C5aR. |
Mendeley readers
The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 6% |
| Unknown | 15 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 19% |
| Other | 2 | 13% |
| Professor > Associate Professor | 2 | 13% |
| Student > Ph. D. Student | 2 | 13% |
| Student > Bachelor | 1 | 6% |
| Other | 2 | 13% |
| Unknown | 4 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 19% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 13% |
| Biochemistry, Genetics and Molecular Biology | 2 | 13% |
| Medicine and Dentistry | 2 | 13% |
| Immunology and Microbiology | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 5 | 31% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 December 2023.
All research outputs
#8,535,472
of 25,374,647 outputs
Outputs from Inflammation
#210
of 1,151 outputs
Outputs of similar age
#14,149
of 41,876 outputs
Outputs of similar age from Inflammation
#1
of 1 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,151 research outputs from this source. They receive a mean Attention Score of 2.9. This one has gotten more attention than average, scoring higher than 63% of its peers.
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