| Title |
Definition of constitutive gene expression in plants: the translation initiation factor 4A gene as a model
|
|---|---|
| Published in |
Plant Molecular Biology, December 1995
|
| DOI | 10.1007/bf00014972 |
| Pubmed ID | |
| Authors |
Therese Mandel, Andrew J. Fleming, Rolf Krähenbühl, Cris Kuhlemeier |
| Abstract |
The NeIF-4A10 gene belongs to a family of at least ten genes, all of which encode closely related isoforms of translation initiation factor 4A. The promoter region of NeIF-4A10 was sequenced, and four mRNA 5' ends were determined. Deletions containing 2750, 689 and 188 bp of untranscribed upstream DNA were fused to the GUS reporter gene and introduced into transgenic tobacco. The three constructs mediated GUS expression in all cells of the leaf, stem and shoot apical meristem. Control experiments using in situ hybridization and tissue printing indicated that the observed GUS expression matches the expression patterns of NeIF-4A mRNA and protein. This detailed analysis at the level of mRNA, protein and reporter gene expression shows that NeIF-4A10 is an ideal constitutively expressed control gene. We argue that inclusion of such a control gene in experiments dealing with specifically expressed genes is in many cases essential for the correct interpretation of observed expression patterns. |
Mendeley readers
The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 4% |
| United States | 1 | 4% |
| Unknown | 21 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 30% |
| Student > Master | 3 | 13% |
| Researcher | 3 | 13% |
| Lecturer > Senior Lecturer | 2 | 9% |
| Professor | 2 | 9% |
| Other | 3 | 13% |
| Unknown | 3 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 12 | 52% |
| Biochemistry, Genetics and Molecular Biology | 5 | 22% |
| Business, Management and Accounting | 1 | 4% |
| Medicine and Dentistry | 1 | 4% |
| Chemistry | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 3 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 September 2022.
All research outputs
#7,680,234
of 23,371,053 outputs
Outputs from Plant Molecular Biology
#985
of 2,858 outputs
Outputs of similar age
#16,666
of 79,541 outputs
Outputs of similar age from Plant Molecular Biology
#25
of 58 outputs
Altmetric has tracked 23,371,053 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,858 research outputs from this source. They receive a mean Attention Score of 4.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 79,541 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 19th percentile – i.e., 19% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 58 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.