| Title |
Rapid detection of filoviruses by real-time TaqMan polymerase chain reaction assays
|
|---|---|
| Published in |
Virologica Sinica, September 2012
|
| DOI | 10.1007/s12250-012-3252-y |
| Pubmed ID | |
| Authors |
Yi Huang, Hongping Wei, Yunpeng Wang, Zhengli Shi, Herve Raoul, Zhiming Yuan |
| Abstract |
Ebola virus (EBOV) and Marburg virus (MARV) are causative agents of severe hemorrhagic fever with high mortality rates in humans and non-human primates and there is currently no licensed vaccine or therapeutics. To date, there is no specific laboratory diagnostic test in China, while there is a national need to provide differential diagnosis during outbreaks and for instituting acceptable quarantine procedures. In this study, the TaqMan RT-PCR assays targeting the nucleoprotein genes of the Zaire Ebolavirus (ZEBOV) and MARV were developed and their sensitivities and specificities were investigated. Our results indicated that the assays were able to make reliable diagnosis over a wide range of virus copies from 10(3) to 10(9), corresponding to the threshold of a standard RNA transcript. The results showed that there were about 10(10) RNA copies per milliliter of virus culture supernatant, equivalent to 10,000 RNA molecules per infectious virion, suggesting the presence of many non-infectious particles. These data indicated that the TaqMan RT-PCR assays developed in this study will be suitable for future surveillance and specific diagnosis of ZEBOV and MARV in China. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 33% |
| Venezuela, Bolivarian Republic of | 1 | 33% |
| United Kingdom | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 69 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 3% |
| United States | 1 | 1% |
| Canada | 1 | 1% |
| Australia | 1 | 1% |
| Unknown | 64 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 17 | 25% |
| Student > Ph. D. Student | 12 | 17% |
| Student > Bachelor | 8 | 12% |
| Other | 6 | 9% |
| Student > Master | 6 | 9% |
| Other | 12 | 17% |
| Unknown | 8 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 22 | 32% |
| Medicine and Dentistry | 17 | 25% |
| Nursing and Health Professions | 4 | 6% |
| Immunology and Microbiology | 4 | 6% |
| Biochemistry, Genetics and Molecular Biology | 3 | 4% |
| Other | 7 | 10% |
| Unknown | 12 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 September 2021.
All research outputs
#13,109,676
of 23,105,443 outputs
Outputs from Virologica Sinica
#208
of 579 outputs
Outputs of similar age
#90,128
of 171,648 outputs
Outputs of similar age from Virologica Sinica
#3
of 4 outputs
Altmetric has tracked 23,105,443 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 579 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 12.0. This one has gotten more attention than average, scoring higher than 62% of its peers.
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