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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Autophagy regulates trans fatty acid-mediated apoptosis in primary cardiac myofibroblasts
|
|---|---|
| Published in |
Biochimica et Biophysica Acta (BBA), September 2012
|
| DOI | 10.1016/j.bbamcr.2012.09.008 |
| Pubmed ID | |
| Authors |
Saeid Ghavami, Ryan H. Cunnington, Behzad Yeganeh, Jared J.L. Davies, Sunil G. Rattan, Krista Bathe, Morvarid Kavosh, Marek J. Los, Darren H. Freed, Thomas Klonisch, Grant N. Pierce, Andrew J. Halayko, Ian M.C. Dixon |
| Abstract |
Trans fats are not a homogeneous group of molecules and less is known about the cellular effects of individual members of the group. Vaccenic acid (VA) and elaidic acid (EA) are the predominant trans monoenes in ruminant fats and vegetable oil, respectively. Here, we investigated the mechanism of cell death induced by VA and EA on primary rat ventricular myofibroblasts (rVF). The MTT assay demonstrated that both VA and EA (200μM, 0-72 h) reduced cell viability in rVF (P<0.001). The FACS assay confirmed that both VA and EA induced apoptosis in rVF, and this was concomitant with elevation in cleaved caspase-9, -3 and -7, but not caspase-8. VA and EA decreased the expression ratio of Bcl2:Bax, induced Bax translocation to mitochondria and decrease in mitochondrial membrane potential (Δψ). BAX and BAX/BAK silencing in mouse embryonic fibroblasts (MEF) inhibited VA and EA-induced cell death compared to the corresponding wild type cells. Transmission electron microscopy revealed that VA and EA also induced macroautophagosome formation in rVF, and immunoblot analysis confirmed the induction of several autophagy markers: LC3-β lipidation, Atg5-12 accumulation, and increased beclin-1. Finally, deletion of autophagy genes, ATG3 and ATG5 significantly inhibited VA and EA-induced cell death (P<0.001). Our findings show for the first time that trans fat acid (TFA) induces simultaneous apoptosis and autophagy in rVF. Furthermore, TFA-induced autophagy is required for this pro-apoptotic effect. Further studies to address the effect of TFA on the heart may reveal significant translational value for prevention of TFA-linked heart disease. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Italy | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 3% |
| Costa Rica | 1 | 3% |
| Unknown | 38 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 13% |
| Student > Doctoral Student | 5 | 13% |
| Professor | 4 | 10% |
| Student > Bachelor | 4 | 10% |
| Researcher | 4 | 10% |
| Other | 11 | 28% |
| Unknown | 7 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 25% |
| Agricultural and Biological Sciences | 10 | 25% |
| Medicine and Dentistry | 4 | 10% |
| Engineering | 4 | 10% |
| Chemistry | 2 | 5% |
| Other | 2 | 5% |
| Unknown | 8 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 October 2012.
All research outputs
#20,656,820
of 25,374,647 outputs
Outputs from Biochimica et Biophysica Acta (BBA)
#17,497
of 19,218 outputs
Outputs of similar age
#149,330
of 191,380 outputs
Outputs of similar age from Biochimica et Biophysica Acta (BBA)
#146
of 170 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
So far Altmetric has tracked 19,218 research outputs from this source. They receive a mean Attention Score of 4.4. This one is in the 5th percentile – i.e., 5% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 191,380 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 10th percentile – i.e., 10% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 170 others from the same source and published within six weeks on either side of this one. This one is in the 8th percentile – i.e., 8% of its contemporaries scored the same or lower than it.