Title |
Infectious alphavirus production from a simple plasmid transfection
|
---|---|
Published in |
Virology Journal, July 2011
|
DOI | 10.1186/1743-422x-8-356 |
Pubmed ID | |
Authors |
Jordan J Steel, Brittney R Henderson, Siddhi BC Lama, Ken E Olson, Brian J Geiss |
Abstract |
We have developed a new method for producing infectious double subgenomic alphaviruses from plasmids transfected into mammalian cells. A double subgenomic Sindbis virus (TE3'2J) was transcribed from a cytomegalovirus PolII promoter, which results in the production of infectious virus. Transfection of as little as 125 ng of plasmid is able to produce 1 × 10(8) plaque forming units/ml (PFU/ml) of infectious virus 48 hours post-transfection. This system represents a more efficient method for producing recombinant Sindbis viruses. |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 28 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 6 | 21% |
Researcher | 5 | 18% |
Student > Ph. D. Student | 4 | 14% |
Student > Bachelor | 3 | 11% |
Student > Doctoral Student | 2 | 7% |
Other | 6 | 21% |
Unknown | 2 | 7% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 11 | 39% |
Biochemistry, Genetics and Molecular Biology | 7 | 25% |
Medicine and Dentistry | 3 | 11% |
Immunology and Microbiology | 2 | 7% |
Social Sciences | 1 | 4% |
Other | 3 | 11% |
Unknown | 1 | 4% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 February 2017.
All research outputs
#4,487,238
of 22,681,577 outputs
Outputs from Virology Journal
#435
of 3,030 outputs
Outputs of similar age
#24,071
of 119,352 outputs
Outputs of similar age from Virology Journal
#5
of 46 outputs
Altmetric has tracked 22,681,577 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,030 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 25.6. This one has done well, scoring higher than 85% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 119,352 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 79% of its contemporaries.
We're also able to compare this research output to 46 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 89% of its contemporaries.