| Title |
Run-on gene transcription in human neocortical nuclei
|
|---|---|
| Published in |
Journal of Molecular Neuroscience, January 1998
|
| DOI | 10.1385/jmn:11:1:67 |
| Pubmed ID | |
| Authors |
Walter J. Lukiw, Hector J. LeBlanc, Larry A. Carver, Donald R. C. McLachlan, Nicolas G. Bazan |
| Abstract |
The incorporation of [alpha-32P]-uridine triphosphate into DNA transcription products was examined in short post-mortem interval (PMI) human brain neocortical nuclei (n, 22; PMI, 0.5-24 h) using run-on-gene transcription. Reverse Northern dot-blot hybridization of newly synthesized RNA against either total cDNA or Alu repetitive DNA indicated that human brain neocortical nuclei of up to 4-h PMI were efficient in incorporating radiolabel into new transcription products, after which there was a graded decline in de novo RNA biosynthetic capacity. To test the effects of 0-3000 nM concentrations of ambient aluminum on RNA polymerase I (RNAP I) and RNA polymerase II (RNAP II) transcription, dot blots containing 0.5, 1.0, 2.0, and 5.0 micrograms of DNA for (1) the human-specific Alu repetitive element (2) the neurofilament light (NFL) chain, and (3) glial fibrillary acidic protein (GFAP) were Northern hybridized against newly synthesized radiolabeled total RNA. These DNAs represent heterogeneous nuclear RNA (hnRNA), neuronal-, and glial-specific markers, respectively. We report here a dose-dependent repression in the biosynthetic capabilities of brain RNAP II in the range of 50-100 nM aluminum, deficits similar to those previously described using a rabbit neocortical nuclei transcription system and at concentrations that have been reported in Alzheimer's disease (AD) euchromatin. Transcription from RNAP II and the neuron-specific NFL gene in the presence of aluminum was found to be particularly affected. These findings support the hypothesis that brain gene transcription in the presence of trace amounts of ambient aluminum impairs mammalian brain DNA to adequately read out genetic information. |
Mendeley readers
The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 24 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 6 | 25% |
| Student > Ph. D. Student | 5 | 21% |
| Other | 3 | 13% |
| Student > Bachelor | 3 | 13% |
| Lecturer | 2 | 8% |
| Other | 3 | 13% |
| Unknown | 2 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 29% |
| Agricultural and Biological Sciences | 2 | 8% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 8% |
| Psychology | 2 | 8% |
| Medicine and Dentistry | 2 | 8% |
| Other | 5 | 21% |
| Unknown | 4 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 July 2016.
All research outputs
#22,759,452
of 25,374,647 outputs
Outputs from Journal of Molecular Neuroscience
#1,330
of 1,643 outputs
Outputs of similar age
#93,278
of 94,808 outputs
Outputs of similar age from Journal of Molecular Neuroscience
#12
of 12 outputs
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