| Title |
Gap Junction Protocols
|
|---|---|
| Published by |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3664-9 |
| Pubmed ID | |
| ISBNs |
978-1-4939-3662-5, 978-1-4939-3664-9
|
| Authors |
Lohman, Alexander W, Straub, Adam C, Johnstone, Scott R, Good, Miranda E, Begandt, Daniela, DeLalio, Leon J, Isakson, Brant E |
| Editors |
Mathieu Vinken, Scott R. Johnstone |
| Abstract |
All connexins (Cx) proteins contain both highly ordered domains (i.e., 4 transmembrane domains) and primarily unstructured regions (i.e., n- and c-terminal domains). The c-terminal domains vary in length and amino acid composition from the shortest on Cx26 to the longest on Cx43. With the exception of Cx26, the c-terminal domains contain multiple sites for posttranslational modification (PTM) including serines (S), threonines (T), and tyrosines (Y) for phosphorylation or cysteines (C) for S-nitrosylation. These PTMs are critical for regulating cellular localization, protein-protein interactions, and channel functionality. There are several biochemical techniques that allow for the identification of these PTM including Western blotting and the "Biotin Switch" assay for nitrosylation. Quantitative analysis of Western blots can be achieved through use of secondary antibodies in the near infrared scale and high-resolution scanning on a fluorescent scanner. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 29% |
| United Kingdom | 1 | 14% |
| Canada | 1 | 14% |
| France | 1 | 14% |
| Unknown | 2 | 29% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 7 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 32 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Lecturer > Senior Lecturer | 1 | 3% |
| Student > Doctoral Student | 1 | 3% |
| Student > Bachelor | 1 | 3% |
| Professor | 1 | 3% |
| Student > Ph. D. Student | 1 | 3% |
| Other | 2 | 6% |
| Unknown | 25 | 78% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 16% |
| Chemistry | 1 | 3% |
| Medicine and Dentistry | 1 | 3% |
| Unknown | 25 | 78% |