Title |
Influence of poly(n‐isopropylacrylamide)–CNT–polyaniline three‐dimensional electrospun microfabric scaffolds on cell growth and viability
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Published in |
Biospectroscopy, February 2013
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DOI | 10.1002/bip.22170 |
Pubmed ID | |
Authors |
Ashutosh Tiwari, Yashpal Sharma, Shinya Hattori, Dohiko Terada, Ashok K. Sharma, Anthony P. F. Turner, Hisatoshi Kobayashi |
Abstract |
This study investigates the effect on: (1) the bulk surface and (2) the three-dimensional non-woven microfabric scaffolds of poly(N-isopropylacrylamide)-CNT-polyaniline on growth and viability of cells. The poly(N-isopropylacrylamide)-CNT-polyaniline was prepared using coupling chemistry and electrospinning was then used for the fabrication of responsive, non-woven microfabric scaffolds. The electrospun microfabrics were assembled in regular three-dimensional scaffolds with OD: 400-500 μm; L: 6-20 cm. Mice fibroblast cells L929 were seeded on the both poly(N-isopropylacrylamide)-CNT-polyaniline bulk surface as well as non-woven microfabric scaffolds. Excellent cell proliferation and viability was observed on poly(N-isopropylacrylamide)-CNT-polyaniline non-woven microfabric matrices in compare to poly(N-isopropylacrylamide)-CNT-polyaniline bulk and commercially available Matrigel™ even with a range of cell lines up to 168 h. Temperature dependent cells detachment behavior was observed on the poly(N-isopropylacrylamide)-CNT-polyaniline scaffolds by varying incubation at below lower critical solution temperature of poly(N-isopropylacrylamide). The results suggest that poly(N-isopropylacrylamide)-CNT-polyaniline non-woven microfabrics could be used as a smart matrices for applications in tissue engineering. © 2012 Wiley Periodicals, Inc. Biopolymers 99: 334-341, 2013. |
X Demographics
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United Kingdom | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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United Kingdom | 1 | 3% |
Mexico | 1 | 3% |
Unknown | 36 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 9 | 24% |
Researcher | 8 | 21% |
Student > Bachelor | 3 | 8% |
Professor | 3 | 8% |
Student > Master | 3 | 8% |
Other | 6 | 16% |
Unknown | 6 | 16% |
Readers by discipline | Count | As % |
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Materials Science | 9 | 24% |
Engineering | 5 | 13% |
Chemistry | 4 | 11% |
Chemical Engineering | 3 | 8% |
Agricultural and Biological Sciences | 2 | 5% |
Other | 6 | 16% |
Unknown | 9 | 24% |