Title |
Using Tablet for visual exploration of second-generation sequencing data
|
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Published in |
Briefings in Bioinformatics, March 2012
|
DOI | 10.1093/bib/bbs012 |
Pubmed ID | |
Authors |
Iain Milne, Gordon Stephen, Micha Bayer, Peter J A Cock, Leighton Pritchard, Linda Cardle, Paul D Shaw, David Marshall |
Abstract |
The advent of second-generation sequencing (2GS) has provided a range of significant new challenges for the visualization of sequence assemblies. These include the large volume of data being generated, short-read lengths and different data types and data formats associated with the diversity of new sequencing technologies. This article illustrates how Tablet-a high-performance graphical viewer for visualization of 2GS assemblies and read mappings-plays an important role in the analysis of these data. We present Tablet, and through a selection of use cases, demonstrate its value in quality assurance and scientific discovery, through features such as whole-reference coverage overviews, variant highlighting, paired-end read mark-up, GFF3-based feature tracks and protein translations. We discuss the computing and visualization techniques utilized to provide a rich and responsive graphical environment that enables users to view a range of file formats with ease. Tablet installers can be freely downloaded from http://bioinf.hutton.ac.uk/tablet in 32 or 64-bit versions for Windows, OS X, Linux or Solaris. For further details on the Tablet, contact [email protected]. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 3 | 27% |
France | 3 | 27% |
Germany | 2 | 18% |
Unknown | 3 | 27% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 7 | 64% |
Scientists | 4 | 36% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 5 | <1% |
Australia | 5 | <1% |
United States | 5 | <1% |
Spain | 4 | <1% |
Canada | 4 | <1% |
United Kingdom | 4 | <1% |
Germany | 2 | <1% |
Mexico | 2 | <1% |
Czechia | 2 | <1% |
Other | 14 | 2% |
Unknown | 578 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 143 | 23% |
Student > Ph. D. Student | 139 | 22% |
Student > Master | 76 | 12% |
Student > Bachelor | 65 | 10% |
Student > Doctoral Student | 28 | 4% |
Other | 90 | 14% |
Unknown | 84 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 292 | 47% |
Biochemistry, Genetics and Molecular Biology | 131 | 21% |
Computer Science | 22 | 4% |
Immunology and Microbiology | 17 | 3% |
Environmental Science | 16 | 3% |
Other | 52 | 8% |
Unknown | 95 | 15% |