| Title |
A Protein‐Based Pentavalent Inhibitor of the Cholera Toxin B‐Subunit
|
|---|---|
| Published in |
Angewandte Chemie. International Edition, July 2014
|
| DOI | 10.1002/anie.201404397 |
| Pubmed ID | |
| Authors |
Thomas R. Branson, Tom E. McAllister, Jaime Garcia‐Hartjes, Martin A. Fascione, James F. Ross, Stuart L. Warriner, Tom Wennekes, Han Zuilhof, W. Bruce Turnbull |
| Abstract |
Protein toxins produced by bacteria are the cause of many life-threatening diarrheal diseases. Many of these toxins, including cholera toxin (CT), enter the cell by first binding to glycolipids in the cell membrane. Inhibiting these multivalent protein/carbohydrate interactions would prevent the toxin from entering cells and causing diarrhea. Here we demonstrate that the site-specific modification of a protein scaffold, which is perfectly matched in both size and valency to the target toxin, provides a convenient route to an effective multivalent inhibitor. The resulting pentavalent neoglycoprotein displays an inhibition potency (IC50) of 104 pM for the CT B-subunit (CTB), which is the most potent pentavalent inhibitor for this target reported thus far. Complexation of the inhibitor and CTB resulted in a protein heterodimer. This inhibition strategy can potentially be applied to many multivalent receptors and also opens up new possibilities for protein assembly strategies. |
X Demographics
The data shown below were collected from the profiles of 13 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 4 | 31% |
| South Africa | 1 | 8% |
| Netherlands | 1 | 8% |
| Unknown | 7 | 54% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 8 | 62% |
| Scientists | 5 | 38% |
Mendeley readers
The data shown below were compiled from readership statistics for 74 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 1% |
| Netherlands | 1 | 1% |
| China | 1 | 1% |
| France | 1 | 1% |
| Unknown | 70 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 21 | 28% |
| Researcher | 14 | 19% |
| Student > Master | 9 | 12% |
| Student > Bachelor | 6 | 8% |
| Professor > Associate Professor | 6 | 8% |
| Other | 7 | 9% |
| Unknown | 11 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 39 | 53% |
| Biochemistry, Genetics and Molecular Biology | 10 | 14% |
| Agricultural and Biological Sciences | 9 | 12% |
| Medicine and Dentistry | 2 | 3% |
| Earth and Planetary Sciences | 1 | 1% |
| Other | 2 | 3% |
| Unknown | 11 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 64. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 September 2022.
All research outputs
#662,849
of 25,394,764 outputs
Outputs from Angewandte Chemie. International Edition
#644
of 50,044 outputs
Outputs of similar age
#6,099
of 242,208 outputs
Outputs of similar age from Angewandte Chemie. International Edition
#11
of 742 outputs
Altmetric has tracked 25,394,764 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 50,044 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.8. This one has done particularly well, scoring higher than 98% of its peers.
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We're also able to compare this research output to 742 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 98% of its contemporaries.