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Modified silver staining in 2DE improves protein detection even at extremely low sample concentration

Overview of attention for article published in Electrophoresis, January 2013
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1 X user

Citations

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Title
Modified silver staining in 2DE improves protein detection even at extremely low sample concentration
Published in
Electrophoresis, January 2013
DOI 10.1002/elps.201200380
Pubmed ID
Authors

Yun Khoon Liew, Vasanthakumari Neela, Rukman Awang Hamat, Syafinaz Amin Nordin, Pei Pei Chong

Abstract

The typical concentration of protein loaded varies from 0.13 to 1.40 μg/μL for a classical silver staining method in 2DE gel. Here, we present a simple modified classical silver staining method by modifying the silver impregnation and development reaction steps. This modified method detects the protein spots at extremely low loaded concentrations, ranging from 0.0048 to 0.0480 μg/μL. We recommend this modified silver staining as an excellent method for the limited biological samples used for silver-stained 2DE analysis. Altogether, the protocol takes close to two days from first dimension separation to second dimension separation, followed by silver staining, scanning, and analysis.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Croatia 1 6%
Unknown 15 94%

Demographic breakdown

Readers by professional status Count As %
Professor 3 19%
Researcher 3 19%
Student > Bachelor 2 13%
Professor > Associate Professor 2 13%
Student > Ph. D. Student 1 6%
Other 3 19%
Unknown 2 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 38%
Medicine and Dentistry 4 25%
Biochemistry, Genetics and Molecular Biology 3 19%
Social Sciences 1 6%
Immunology and Microbiology 1 6%
Other 0 0%
Unknown 1 6%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 November 2012.
All research outputs
#20,039,697
of 24,629,540 outputs
Outputs from Electrophoresis
#3,047
of 3,904 outputs
Outputs of similar age
#228,770
of 290,892 outputs
Outputs of similar age from Electrophoresis
#27
of 128 outputs
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