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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Cloning, expression and characterization of a novel cold-adapted GDSL family esterase from Photobacterium sp. strain J15
|
|---|---|
| Published in |
Extremophiles, October 2015
|
| DOI | 10.1007/s00792-015-0796-4 |
| Pubmed ID | |
| Authors |
Mehrnoush Hadaddzadeh Shakiba, Mohd Shukuri Mohamad Ali, Raja Noor Zaliha Raja Abd Rahman, Abu Bakar Salleh, Thean Chor Leow |
| Abstract |
The gene encoding for a novel cold-adapted enzyme from family II of bacterial classification (GDSL family) was cloned from the genomic DNA of Photobacterium sp. strain J15 in an Escherichia coli system, yielding a recombinant 36 kDa J15 GDSL esterase which was purified in two steps with a final yield and purification of 38.6 and 15.3 respectively. Characterization of the biochemical properties showed the J15 GDSL esterase had maximum activity at 20 °C and pH 8.0, was stable at 10 °C for 3 h and retained 50 % of its activity after a 6 h incubation at 10 °C. The enzyme was activated by Tween-20, -60 and Triton-X100 and inhibited by 1 mM Sodium dodecyl sulphate (SDS), while β-mercaptoethanol and Dithiothreitol (DTT) enhanced activity by 4.3 and 5.4 fold respectively. These results showed the J15 GDSL esterase was a novel cold-adapted enzyme from family II of lipolytic enzymes. A structural model constructed using autotransporter EstA from Pseudomonas aeruginosa as a template revealed the presence of a typical catalytic triad consisting of a serine, aspartate, and histidine which was verified with site directed mutagenesis on active serine. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 46 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 1 | 2% |
| Unknown | 45 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 17% |
| Student > Bachelor | 7 | 15% |
| Professor | 5 | 11% |
| Student > Postgraduate | 5 | 11% |
| Student > Master | 5 | 11% |
| Other | 8 | 17% |
| Unknown | 8 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 14 | 30% |
| Agricultural and Biological Sciences | 13 | 28% |
| Engineering | 3 | 7% |
| Veterinary Science and Veterinary Medicine | 2 | 4% |
| Environmental Science | 1 | 2% |
| Other | 2 | 4% |
| Unknown | 11 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 October 2015.
All research outputs
#15,348,897
of 22,830,751 outputs
Outputs from Extremophiles
#543
of 799 outputs
Outputs of similar age
#164,093
of 280,050 outputs
Outputs of similar age from Extremophiles
#5
of 14 outputs
Altmetric has tracked 22,830,751 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 799 research outputs from this source. They receive a mean Attention Score of 4.2. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 280,050 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 14 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.