Title |
MALDI-target integrated platform for affinity-captured protein digestion
|
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Published in |
Analytica Chimica Acta, September 2013
|
DOI | 10.1016/j.aca.2013.08.051 |
Pubmed ID | |
Authors |
Asilah Ahmad-Tajudin, Belinda Adler, Simon Ekström, György Marko-Varga, Johan Malm, Hans Lilja, Thomas Laurell |
Abstract |
To address immunocapture of proteins in large cohorts of clinical samples high throughput sample processing is required. Here a method using the proteomic sample platform, ISET (integrated selective enrichment target) that integrates highly specific immunoaffinity capture of protein biomarker, digestion and sample cleanup with a direct interface to mass spectrometry is presented. The robustness of the on-ISET protein digestion protocol was validated by MALDI MS analysis of model proteins, ranging from 40 fmol to 1 pmol per nanovial. On-ISET digestion and MALDI MS/MS analysis of immunoaffinity captured disease-associated biomarker PSA (prostate specific antigen) from human seminal plasma are presented. |
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Demographic breakdown
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Scientists | 1 | 100% |
Mendeley readers
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Demographic breakdown
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Student > Bachelor | 4 | 13% |
Researcher | 4 | 13% |
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Student > Postgraduate | 3 | 10% |
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