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Mendeley readers
Attention Score in Context
| Title |
Purification of human adipose-derived stem cells from fat tissues using PLGA/silk screen hybrid membranes
|
|---|---|
| Published in |
Clinical Materials, February 2014
|
| DOI | 10.1016/j.biomaterials.2014.02.004 |
| Pubmed ID | |
| Authors |
Da-Chung Chen, Li-Yu Chen, Qing-Dong Ling, Meng-Hsueh Wu, Ching-Tang Wang, S. Suresh Kumar, Yung Chang, Murugan A. Munusamy, Abdullah A. Alarfajj, Han-Chow Wang, Shih-Tien Hsu, Akon Higuchi |
| Abstract |
The purification of human adipose-derived stem cells (hADSCs) from human adipose tissue cells (stromal vascular fraction) was investigated using membrane filtration through poly(lactide-co-glycolic acid)/silk screen hybrid membranes. Membrane filtration methods are attractive in regenerative medicine because they reduce the time required to purify hADSCs (i.e., less than 30 min) compared with conventional culture methods, which require 5-12 days. hADSCs expressing the mesenchymal stem cell markers CD44, CD73, and CD90 were concentrated in the permeation solution from the hybrid membranes. Expression of the surface markers CD44, CD73, and CD99 on the cells in the permeation solution from the hybrid membranes, which were obtained using 18 mL of feed solution containing 50 × 10⁴ cells, was statistically significantly higher than that of the primary adipose tissue cells, indicating that the hADSCs can be purified in the permeation solution by the membrane filtration method. Cells expressing the stem cell-associated marker CD34 could be successfully isolated in the permeation solution, whereas CD34⁺ cells could not be purified by the conventional culture method. The hADSCs in the permeation solution demonstrated a superior capacity for osteogenic differentiation based on their alkali phosphatase activity, their osterix gene expression, and the results of mineralization analysis by Alizarin Red S and von Kossa staining compared with the cells from the suspension of human adipose tissue. These results suggest that the hADSCs capable of osteogenic differentiation preferentially permeate through the hybrid membranes. |
Mendeley readers
The data shown below were compiled from readership statistics for 49 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 2% |
| Unknown | 48 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 16% |
| Student > Master | 8 | 16% |
| Researcher | 7 | 14% |
| Other | 6 | 12% |
| Student > Bachelor | 5 | 10% |
| Other | 9 | 18% |
| Unknown | 6 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 13 | 27% |
| Medicine and Dentistry | 10 | 20% |
| Biochemistry, Genetics and Molecular Biology | 8 | 16% |
| Chemistry | 2 | 4% |
| Environmental Science | 2 | 4% |
| Other | 8 | 16% |
| Unknown | 6 | 12% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 April 2019.
All research outputs
#4,922,392
of 25,707,225 outputs
Outputs from Clinical Materials
#2,046
of 10,812 outputs
Outputs of similar age
#45,872
of 240,559 outputs
Outputs of similar age from Clinical Materials
#39
of 142 outputs
Altmetric has tracked 25,707,225 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 10,812 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.9. This one has done well, scoring higher than 79% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 240,559 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 79% of its contemporaries.
We're also able to compare this research output to 142 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.