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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Continuous harvest of stem cells via partial detachment from thermoresponsive nanobrush surfaces
|
|---|---|
| Published in |
Clinical Materials, October 2015
|
| DOI | 10.1016/j.biomaterials.2015.10.039 |
| Pubmed ID | |
| Authors |
I-Chia Peng, Chin-Chen Yeh, Yi-Tung Lu, Saradaprasan Muduli, Qing-Dong Ling, Abdullah A. Alarfaj, Murugan A. Munusamy, S. Suresh Kumar, Kadarkarai Murugan, Hsin-chung Lee, Yung Chang, Akon Higuchi |
| Abstract |
Stem cell culture is typically based on batch-type culture, which is laborious and expensive. Here, we propose a continuous harvest method for stem cells cultured on thermoresponsive nanobrush surfaces. In this method, stem cells are partially detached from the nanobrush surface by reducing the temperature of the culture medium below the critical solution temperature needed for thermoresponse. The detached stem cells are harvested by exchange into fresh culture medium. Following this, the remaining cells are continuously cultured by expansion in fresh culture medium at 37 °C. Thermoresponsive nanobrush surfaces were prepared by coating block copolymers containing polystyrene (for hydrophobic anchoring onto culture dishes) with three types of polymers: (a) polyacrylic acid with cell-binding oligopeptides, (b) thermoresponsive poly-N-isopropylacrylamide, and (c) hydrophilic poly(ethyleneglycol)methacrylate. The optimal coating durations and compositions for these copolymers to facilitate adequate attachment and detachment of human adipose-derived stem cells (hADSCs) and embryonic stem cells (hESCs) were determined. hADSCs and hESCs were continuously harvested for 5 and 3 cycles, respectively, via the partial detachment of cells from thermoresponsive nanobrush surfaces. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 2 | 4% |
| Unknown | 43 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 15 | 33% |
| Student > Bachelor | 7 | 16% |
| Student > Master | 5 | 11% |
| Researcher | 3 | 7% |
| Student > Doctoral Student | 2 | 4% |
| Other | 6 | 13% |
| Unknown | 7 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 7 | 16% |
| Medicine and Dentistry | 7 | 16% |
| Biochemistry, Genetics and Molecular Biology | 6 | 13% |
| Engineering | 5 | 11% |
| Agricultural and Biological Sciences | 4 | 9% |
| Other | 7 | 16% |
| Unknown | 9 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 November 2015.
All research outputs
#17,313,103
of 25,411,814 outputs
Outputs from Clinical Materials
#9,093
of 10,758 outputs
Outputs of similar age
#176,287
of 294,186 outputs
Outputs of similar age from Clinical Materials
#96
of 134 outputs
Altmetric has tracked 25,411,814 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 10,758 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.8. This one is in the 11th percentile – i.e., 11% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 294,186 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 134 others from the same source and published within six weeks on either side of this one. This one is in the 22nd percentile – i.e., 22% of its contemporaries scored the same or lower than it.