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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Sensitive detection of multiple pathogens using a single DNA probe
|
|---|---|
| Published in |
Biosensors & Bioelectronics, June 2016
|
| DOI | 10.1016/j.bios.2016.06.077 |
| Pubmed ID | |
| Authors |
Noordiana Nordin, Nor Azah Yusof, Jaafar Abdullah, Son Radu, Roozbeh Hushiarian |
| Abstract |
A simple but promising electrochemical DNA nanosensor was designed, constructed and applied to differentiate a few food-borne pathogens. The DNA probe was initially designed to have a complementary region in Vibrio parahaemolyticus (VP) genome and to make different hybridization patterns with other selected pathogens. The sensor was based on a screen printed carbon electrode (SPCE) modified with polylactide-stabilized gold nanoparticles (PLA-AuNPs) and methylene blue (MB) was employed as the redox indicator binding better to single-stranded DNA. The immobilization and hybridization events were assessed using differential pulse voltammetry (DPV). The fabricated biosensor was able to specifically distinguish complementary, non-complementary and mismatched oligonucleotides. DNA was measured in the range of 2.0×10(-9)-2.0×10(-13)M with a detection limit of 5.3×10(-12)M. The relative standard deviation for 6 replications of DPV measurement of 0.2µM complementary DNA was 4.88%. The fabricated DNA biosensor was considered stable and portable as indicated by a recovery of more than 80% after a storage period of 6 months at 4-45°C. Cross-reactivity studies against various food-borne pathogens showed a reliably sensitive detection of VP. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 67 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Iran, Islamic Republic of | 1 | 1% |
| Australia | 1 | 1% |
| Unknown | 65 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 15 | 22% |
| Researcher | 9 | 13% |
| Student > Master | 7 | 10% |
| Student > Doctoral Student | 5 | 7% |
| Student > Bachelor | 5 | 7% |
| Other | 13 | 19% |
| Unknown | 13 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 12 | 18% |
| Biochemistry, Genetics and Molecular Biology | 9 | 13% |
| Agricultural and Biological Sciences | 7 | 10% |
| Engineering | 3 | 4% |
| Environmental Science | 3 | 4% |
| Other | 13 | 19% |
| Unknown | 20 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 July 2016.
All research outputs
#15,740,207
of 25,374,917 outputs
Outputs from Biosensors & Bioelectronics
#4,248
of 6,847 outputs
Outputs of similar age
#210,235
of 367,736 outputs
Outputs of similar age from Biosensors & Bioelectronics
#57
of 145 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 6,847 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.6. This one is in the 36th percentile – i.e., 36% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 367,736 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 41st percentile – i.e., 41% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 145 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 60% of its contemporaries.