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Polymerase chain reaction assay targeting cytochrome b gene for the detection of dog meat adulteration in meatball formulation

Overview of attention for article published in Meat Science, March 2014
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1 X user

Citations

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154 Mendeley
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Title
Polymerase chain reaction assay targeting cytochrome b gene for the detection of dog meat adulteration in meatball formulation
Published in
Meat Science, March 2014
DOI 10.1016/j.meatsci.2014.03.011
Pubmed ID
Authors

Mahfujur Rahman, Eaqub Ali, Sharifah Bee Abd Hamid, Shuhaimi Mustafa, Uda Hashim, Ummi Kalthum Hanapi

Abstract

A polymerase chain reaction (PCR) assay for the assessment of dog meat adulteration in meatballs was developed. The assay selectively amplified a 100-bp region of canine mitochondrial cytochrome b gene from pure, raw, processed and mixed backgrounds. The specificity of the assay was tested against 11 animals and 3 plants species, commonly available for meatball formulation. The stability of the assay was proven under extensively autoclaving conditions that breakdown target DNA. A blind test from ready to eat chicken and beef meatballs showed that the assay can repeatedly detect 0.2% canine meat tissues under complex matrices using 0.04 ng of dog DNA extracted from differentially treated meatballs. The simplicity, stability and sensitivity of the assay suggested that it could be used in halal food industry for the authentication of canine derivatives in processed foods.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 154 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 <1%
Colombia 1 <1%
Unknown 152 99%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 22 14%
Student > Master 18 12%
Researcher 14 9%
Lecturer 14 9%
Student > Ph. D. Student 10 6%
Other 21 14%
Unknown 55 36%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 21 14%
Agricultural and Biological Sciences 21 14%
Chemistry 13 8%
Engineering 7 5%
Economics, Econometrics and Finance 6 4%
Other 28 18%
Unknown 58 38%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 February 2015.
All research outputs
#17,286,645
of 25,374,917 outputs
Outputs from Meat Science
#1,186
of 1,781 outputs
Outputs of similar age
#142,924
of 238,075 outputs
Outputs of similar age from Meat Science
#10
of 12 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,781 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one is in the 23rd percentile – i.e., 23% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 238,075 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one is in the 8th percentile – i.e., 8% of its contemporaries scored the same or lower than it.