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Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods

Overview of attention for article published in Scientific Reports, January 2017
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Title
Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods
Published in
Scientific Reports, January 2017
DOI 10.1038/srep40069
Pubmed ID
Authors

Hong Reng Lin, Chao-Wen Heish, Cheng-Hui Liu, Saradaprasan Muduli, Hsing-Fen Li, Akon Higuchi, S. Suresh Kumar, Abdullah A. Alarfaj, Murugan A. Munusamy, Shih-Tien Hsu, Da-Chung Chen, Giovanni Benelli, Kadarkarai Murugan, Nai-Chen Cheng, Han-Chow Wang, Gwo-Jang Wu

Abstract

Human adipose-derived stem cells (hADSCs) are easily isolated from fat tissue without ethical concerns, but differ in purity, pluripotency, differentiation ability, and stem cell marker expression, depending on the isolation method. We isolated hADSCs from a primary fat tissue solution using: (1) conventional culture, (2) a membrane filtration method, (3) a membrane migration method where the primary cell solution was permeated through membranes, adhered hADSCs were cultured, and hADSCs migrated out from the membranes. Expression of mesenchymal stem cell markers and pluripotency genes, and osteogenic differentiation were compared for hADSCs isolated by different methods using nylon mesh filter membranes with pore sizes ranging from 11 to 80 μm. hADSCs isolated by the membrane migration method had the highest MSC surface marker expression and efficient differentiation into osteoblasts. Osteogenic differentiation ability of hADSCs and MSC surface marker expression were correlated, but osteogenic differentiation ability and pluripotent gene expression were not.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 52 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 10 19%
Student > Ph. D. Student 7 13%
Student > Bachelor 7 13%
Researcher 4 8%
Student > Doctoral Student 3 6%
Other 9 17%
Unknown 12 23%
Readers by discipline Count As %
Agricultural and Biological Sciences 11 21%
Medicine and Dentistry 10 19%
Biochemistry, Genetics and Molecular Biology 7 13%
Engineering 2 4%
Mathematics 1 2%
Other 5 10%
Unknown 16 31%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 January 2017.
All research outputs
#18,510,888
of 22,931,367 outputs
Outputs from Scientific Reports
#93,726
of 123,854 outputs
Outputs of similar age
#311,589
of 421,506 outputs
Outputs of similar age from Scientific Reports
#2,873
of 3,809 outputs
Altmetric has tracked 22,931,367 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 123,854 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 18.2. This one is in the 14th percentile – i.e., 14% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 421,506 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 3,809 others from the same source and published within six weeks on either side of this one. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.