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Mendeley readers
| Title |
Crystallization and X‐ray crystallographic analysis of recombinant TylP, a putative γ‐butyrolactone receptor protein from Streptomyces fradiae
|
|---|---|
| Published in |
Acta Crystallographica Section F: Structural Biology Communications, January 2017
|
| DOI | 10.1107/s2053230x17001212 |
| Pubmed ID | |
| Authors |
Nurhikmah Mohd-Sharif, Sofiyah Shaibullah, Vasanthakumar Givajothi, Cheng-Seng Tan, Kok Lian Ho, Aik-Hong Teh, Syarul Nataqain Baharum, Jitka Waterman, Chyan Leong Ng |
| Abstract |
TylP is one of five regulatory proteins involved in the regulation of antibiotic (tylosin) production, morphological and physiological differentiation in Streptomyces fradiae. Its function is similar to those of various γ-butyrolactone receptor proteins. In this report, N-terminally His-tagged recombinant TylP protein (rTylP) was overproduced in Escherichia coli and purified to homogeneity. The rTylP protein was crystallized from a reservoir solution comprising 34%(v/v) ethylene glycol and 5%(v/v) glycerol. The protein crystals diffracted X-rays to 3.05 Å resolution and belonged to the trigonal space group P3121, with unit-cell parameters a = b = 126.62, c = 95.63 Å. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Lecturer > Senior Lecturer | 3 | 23% |
| Student > Ph. D. Student | 3 | 23% |
| Student > Bachelor | 3 | 23% |
| Student > Doctoral Student | 1 | 8% |
| Student > Master | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 1 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 46% |
| Agricultural and Biological Sciences | 2 | 15% |
| Chemistry | 2 | 15% |
| Immunology and Microbiology | 1 | 8% |
| Unknown | 2 | 15% |