Title |
Changes in rumen protozoal community by condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid in vitro
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Published in |
Journal of Applied Microbiology, June 2017
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DOI | 10.1111/jam.13477 |
Pubmed ID | |
Authors |
M. Saminathan, H.M. Gan, N. Abdullah, C.M.V.L. Wong, S.K. Ramiah, H.Y. Tan, C.C. Sieo, Y.W. Ho |
Abstract |
To evaluate the effects of condensed tannins (CTs) fractions of differing molecular weights (MWs) from a Leucaena leucocephala hybrid-Rendang (LLR) on the rumen protozoal community in vitro. The effects of unfractionated CTs (F0) and CT fractions of different MWs (F1 > F2 > F3 > F4 > F5) on protozoal population and community were evaluated in vitro using rumen microbes and ground guinea grass as the substrate. Higher-MW CT fractions F1 and F2 significantly (P < 0.05) decrease the number of ciliate protozoa. The real-time PCR analysis showed that the total protozoa was significantly (P < 0.05) lower in F0 and all CTs with fractions F1 and F2 having the lowest value. High-throughput sequencing of the partial 18S rRNA gene showed that the genus Entodinium significantly (P < 0.05) decreased with increasing MWs of CT, whereas Anoplodinium-Diplodinium were significantly (P < 0.05) increased. Inclusion of the highest MW CT fraction F1 decreased the relative abundance of the minor genera such as Eudiplodinium and Polyplastron compared to the control and CT fractions F2-F5. CTs of differing MWs could reduce and alter the rumen protozoa population in vitro. This effect was more pronounced for higher-MW CTs. This article is protected by copyright. All rights reserved. |
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