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Changes in rumen protozoal community by condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid in vitro

Overview of attention for article published in Journal of Applied Microbiology, June 2017
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Title
Changes in rumen protozoal community by condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid in vitro
Published in
Journal of Applied Microbiology, June 2017
DOI 10.1111/jam.13477
Pubmed ID
Authors

M. Saminathan, H.M. Gan, N. Abdullah, C.M.V.L. Wong, S.K. Ramiah, H.Y. Tan, C.C. Sieo, Y.W. Ho

Abstract

To evaluate the effects of condensed tannins (CTs) fractions of differing molecular weights (MWs) from a Leucaena leucocephala hybrid-Rendang (LLR) on the rumen protozoal community in vitro. The effects of unfractionated CTs (F0) and CT fractions of different MWs (F1 > F2 > F3 > F4 > F5) on protozoal population and community were evaluated in vitro using rumen microbes and ground guinea grass as the substrate. Higher-MW CT fractions F1 and F2 significantly (P < 0.05) decrease the number of ciliate protozoa. The real-time PCR analysis showed that the total protozoa was significantly (P < 0.05) lower in F0 and all CTs with fractions F1 and F2 having the lowest value. High-throughput sequencing of the partial 18S rRNA gene showed that the genus Entodinium significantly (P < 0.05) decreased with increasing MWs of CT, whereas Anoplodinium-Diplodinium were significantly (P < 0.05) increased. Inclusion of the highest MW CT fraction F1 decreased the relative abundance of the minor genera such as Eudiplodinium and Polyplastron compared to the control and CT fractions F2-F5. CTs of differing MWs could reduce and alter the rumen protozoa population in vitro. This effect was more pronounced for higher-MW CTs. This article is protected by copyright. All rights reserved.

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Mendeley readers

The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 34 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 6 18%
Researcher 6 18%
Student > Bachelor 4 12%
Student > Doctoral Student 2 6%
Other 2 6%
Other 6 18%
Unknown 8 24%
Readers by discipline Count As %
Agricultural and Biological Sciences 16 47%
Biochemistry, Genetics and Molecular Biology 2 6%
Pharmacology, Toxicology and Pharmaceutical Science 1 3%
Unspecified 1 3%
Nursing and Health Professions 1 3%
Other 3 9%
Unknown 10 29%