The aims of this study are to identify the Fusarium isolates based on translation elongation factor (tef) 1α sequence, to determine the genetic diversity among isolates and species using selected microsatellite markers, and to examine the pathogenicity of Fusarium isolates causing fruit rot disease of banana.
One-hundred thirteen microfungi isolates were obtained from fruit rot infected banana in Peninsular Malaysia. However, this study was focused on the dominant number of the discovered microfungi that belongs to the genus Fusarium. There were 48 isolates of the microfungi have been identified belonging to 11 species of Fusarium namely Fusarium incarnatum, Fusarium equiseti, Fusarium camptoceras, Fusarium solani, Fusarium concolor, Fusarium oxysporum, Fusarium proliferatum, Fusarium verticillioides, Fusarium sacchari, Fusarium concentricum, and Fusarium fujikuroi. All Fusarium isolates were grouped into their respective clades indicating their similarities and differences in genetic diversity among isolates. Out of 48 Fusarium isolates tested, 42 isolates causing the fruit rot symptom at different levels of severity based on Disease Severity Index (DSI). The most virulent isolate was F. proliferatum B2433B with DSI of 100%.
All the isolated Fusarium species were successfully identified with some of them were confirmed as the causal agents of pre- and post-harvest fruit rot in banana across Peninsular Malaysia. This article is protected by copyright. All rights reserved.