Title |
Isolation and identification of biocellulose‐producing bacterial strains from Malaysian acidic fruits
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Published in |
Letters in Applied Microbiology, April 2016
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DOI | 10.1111/lam.12568 |
Pubmed ID | |
Authors |
W.W.Y. Voon, Y. Rukayadi, A.S. Meor Hussin |
Abstract |
Biocellulose (BC) is pure extracellular cellulose produced by several species of microorganisms that has numerous applications in the food, biomedical and paper industries. However, the existing biocellulose-producing bacterial strain with high yield was limited. The aim of this study was to isolate and identify the potential biocellulose-producing bacterial isolates from Malaysian acidic fruits. 193 bacterial isolates were obtained from 19 local acidic fruits collected in Malaysia and screened for their ability to produce biocellulose. A total of 15 potential bacterial isolates were then cultured in standard Hestrin-Schramm (HS) medium statically at 30°C for two weeks to determine the biocellulose production. The most potent bacterial isolates were identified using 16S rRNA gene sequence analysis, morphological and biochemical characteristics. Three new and potent biocellulose-producing bacterial strains were isolated from soursop fruit and identified as Stenotrophomonas maltophilia WAUPM42, Pantoea vagans WAUPM45 and Beijerinckia fluminensis WAUPM53. Stenotrophomonas maltophilia WAUPM42 was the most potent biocellulose-producing bacterial strain that produced the highest amount of biocellulose 0.58 g l(-1) in standard HS medium. Whereas, the isolates Pantoea vagans WAUPM45 and Beijerinckia fluminensis WAUPM53 showed 0.50 g l(-1) and 0.52 g l(-1) of biocellulose production, respectively. This article is protected by copyright. All rights reserved. |
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