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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Improvement of isolated caprine islet survival and functionality in vitro by enhancing of PDX1 gene expression
|
|---|---|
| Published in |
Xenotransplantation, April 2017
|
| DOI | 10.1111/xen.12302 |
| Pubmed ID | |
| Authors |
Homayoun Hani, Zeenathul Nazariah Allaudin, Mohd‐Azmi Mohd‐Lila, Kazhal Sarsaifi, Mina Rasouli, Yew Joon Tam, Tengku‐Azmi Tengku‐Ibrahim, Abas Mazni Othman |
| Abstract |
Dead islets replaced with viable islets are a promising offer to restore normal insulin production to a person with diabetes. The main reason for establishing a new islet source for transplantation is the insufficiency of human donor pancreas while using xenogeneic islets perhaps assists this problem. The expression of PDX1 is essential for the pancreas expansion. In mature β-cells, PDX1 has several critical roles such as glucose sensing, insulin synthesis, and insulin secretion. In this study, we aimed to evaluate the expression of pancreatic duodenal homeobox-1 (PDX1) in treated caprine islets in culture and to assess the protective effects of antioxidant factors on the PDX1 gene in cultured caprine islets. Purified islets were treated with serum-free, serum, IBMX, tocopherol, or IBMX and tocopherol media. Quantitative polymerase chain reaction and Western blotting were carried out to compare the expression levels of PDX1 in treated purified islets cultured with different media. Islets treated with IBMX/tocopherol exhibited the highest fold change in the relative expression of PDX1 on day 5 post-treatment (relative expression: 6.80±2.08), whereas serum-treated islets showed the lowest fold changes in PDX1 expression on day 5 post-treatment (0.67±0.36), as compared with the expression on day 1 post-treatment. Insulin production and viability tests of purified islets showed superiority of islet at supplemented serum-free media with IBMX/tocopherol compared to other cultures (53.875%±1.59%). Our results indicated that supplemented serum-free medium with tocopherol and IBMX enhances viability and PDX1 gene expression compared to serum-added and serum-free media. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 33% |
| Professor | 2 | 22% |
| Student > Bachelor | 1 | 11% |
| Student > Master | 1 | 11% |
| Student > Ph. D. Student | 1 | 11% |
| Other | 0 | 0% |
| Unknown | 1 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 33% |
| Biochemistry, Genetics and Molecular Biology | 2 | 22% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 11% |
| Immunology and Microbiology | 1 | 11% |
| Unknown | 2 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 March 2018.
All research outputs
#19,922,330
of 24,484,013 outputs
Outputs from Xenotransplantation
#470
of 567 outputs
Outputs of similar age
#242,035
of 314,492 outputs
Outputs of similar age from Xenotransplantation
#5
of 12 outputs
Altmetric has tracked 24,484,013 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
So far Altmetric has tracked 567 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.6. This one is in the 9th percentile – i.e., 9% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 314,492 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one is in the 33rd percentile – i.e., 33% of its contemporaries scored the same or lower than it.