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High Genetic Diversity of Enterococcus faecium and Enterococcus faecalis Clinical Isolates by Pulsed-Field Gel Electrophoresis and Multilocus Sequence Typing from a Hospital in Malaysia

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Title
High Genetic Diversity of Enterococcus faecium and Enterococcus faecalis Clinical Isolates by Pulsed-Field Gel Electrophoresis and Multilocus Sequence Typing from a Hospital in Malaysia
Published in
BioMed Research International, May 2013
DOI 10.1155/2013/938937
Pubmed ID
Authors

Poh Leng Weng, Ramliza Ramli, Mariana Nor Shamsudin, Yoke-Kqueen Cheah, Rukman Awang Hamat

Abstract

Little is known on the genetic relatedness and potential dissemination of particular enterococcal clones in Malaysia. We studied the antibiotic susceptibility profiles of Enterococcus faecium and Enterococcus faecalis and subjected them to pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). E. faecium and E. faecalis displayed 27 and 30 pulsotypes, respectively, and 10 representative E. faecium and E. faecalis isolates (five each) yielded few different sequence types (STs): ST17 (2 isolates), ST78, ST203, and ST601 for E. faecium, and ST6, ST16, ST28, ST179, and ST399 for E. faecalis. Resistance to tazobactam-piperacillin and ampicillin amongst E. faecium isolates was highly observed as compared to E. faecalis isolates. All of the isolates were sensitive to vancomycin and teicoplanin. The presence of epidemic and nosocomial strains of selected E. faecium STs: 17, 78, and 203 and E. faecalis ST6 as well as high rates of resistance to multiple antibiotics amongst E. faecium isolates is of a particular concern.

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Mendeley readers

The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 45 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 12 27%
Student > Ph. D. Student 6 13%
Student > Bachelor 5 11%
Researcher 5 11%
Student > Doctoral Student 3 7%
Other 8 18%
Unknown 6 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 15 33%
Biochemistry, Genetics and Molecular Biology 8 18%
Immunology and Microbiology 7 16%
Medicine and Dentistry 3 7%
Veterinary Science and Veterinary Medicine 1 2%
Other 3 7%
Unknown 8 18%