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Molecular Relatedness of Methicillin-Resistant S. aureus Isolates from Staff, Environment and Pets at University Veterinary Hospital in Malaysia

Overview of attention for article published in PLOS ONE, August 2012
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Title
Molecular Relatedness of Methicillin-Resistant S. aureus Isolates from Staff, Environment and Pets at University Veterinary Hospital in Malaysia
Published in
PLOS ONE, August 2012
DOI 10.1371/journal.pone.0043329
Pubmed ID
Authors

Erkihun Aklilu, Zunita Zakaria, Latiffah Hassan, Chen Hui Cheng

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) has emerged as a problem in veterinary medicine and is no longer considered as a mere nosocomial pathogen. We studied the occurrence of MRSA in veterinary personnel, cats and dogs and the environmental premises in University Veterinary Hospital (UVH). We found the prevalence of MRSA as follows: UVH 2/28 (7.1%) staff, 8/100 (8%) of the pets [5/50 (10%) of the dogs and 3/50 (6%) of the cats)], and 9/28 (4.5%) of the environmental samples. Antibiotic sensitivity tests (AST) show multi-resistance characteristics of the MRSA and the minimum inhibitory concentration (MIC) values for the isolates ranged from 1.5 µg to >256 µg/ml. Molecular typing by using multi-locus sequence typing (MLST), staphylococcal protein A typing (spa typing) and pulsed-field gel electrophoresis (PFGE) was conducted and the results from MLST indicated that an isolate from a veterinary personnel (PG21), typed as ST1241 belonged to the same clonal complex (CC) as the two isolates from two dogs (DG16 and DG20), both being typed as ST59. The PFGE results revealed that the two isolates from two veterinary personnel, PG21 and PG16 belonged to closely related MRSA strains with isolates from dog (DG36) and from environmental surface (EV100) respectively. The fact that PFGE revealed close similarity between isolates from humans, a dog and environmental surfaces indicates the possibility for either of them to be the source of MRSA and the potential routes and risks of spread.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 1 2%
Kenya 1 2%
Nigeria 1 2%
Unknown 40 93%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 16%
Student > Master 7 16%
Student > Bachelor 4 9%
Lecturer 4 9%
Student > Doctoral Student 3 7%
Other 11 26%
Unknown 7 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 11 26%
Medicine and Dentistry 6 14%
Veterinary Science and Veterinary Medicine 5 12%
Immunology and Microbiology 4 9%
Environmental Science 3 7%
Other 7 16%
Unknown 7 16%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 April 2013.
All research outputs
#18,337,420
of 22,708,120 outputs
Outputs from PLOS ONE
#154,134
of 193,897 outputs
Outputs of similar age
#129,760
of 169,381 outputs
Outputs of similar age from PLOS ONE
#3,429
of 4,365 outputs
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