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Food assimilated by two sympatric populations of the brown planthopper Nilaparvata lugens (Delphacidae) feeding on different host plants contaminates insect DNA detected by RAPD-PCR analysis

Overview of attention for article published in Genetics and Molecular Research, January 2012
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Title
Food assimilated by two sympatric populations of the brown planthopper Nilaparvata lugens (Delphacidae) feeding on different host plants contaminates insect DNA detected by RAPD-PCR analysis
Published in
Genetics and Molecular Research, January 2012
DOI 10.4238/2012.january.9.4
Pubmed ID
Authors

M A Latif, M Y Omar, S G Tan, S S Siraj, M E Ali, M Y Rafii

Abstract

Contamination of insect DNA for RAPD-PCR analysis can be a problem because many primers are non-specific and DNA from parasites or gut contents may be simultaneously extracted along with that of the insect. We measured the quantity of food ingested and assimilated by two sympatric populations of brown planthopper (BPH), Nilaparvata lugens, one from rice and the other from Leersia hexandra (Poaceae), a wetland forage grass, and we also investigated whether host plant DNA contaminates that of herbivore insects in extractions of whole insects. Ingestion and assimilation of food were reduced significantly when individuals derived from one host plant were caged on the other species. The bands, OPA3 (1.25), OPD3 (1.10), OPD3 (0.80), OPD3 (0.60), pUC/M13F (0.35), pUC/M13F (0.20), BOXAIR (0.50), peh#3 (0.50), and peh#3 (0.17) were found in both rice-infesting populations of brown planthopper and its host plant (rice). Similarly, the bands, OPA4 (1.00), OPB10 (0.70), OPD3 (0.90), OPD3 (0.80), OPD3 (0.60), pUC/ M13F (0.35), pUC/M13F (0.20), and BOXAIR (0.50) were found in both Leersia-infesting populations of brown planthopper and the host plant. So, it is clear that the DNA bands amplified in the host plants were also found in the extracts from the insects feeding on them.

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Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Malaysia 1 4%
Unknown 23 96%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 29%
Student > Ph. D. Student 7 29%
Professor 3 13%
Student > Master 2 8%
Student > Bachelor 1 4%
Other 1 4%
Unknown 3 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 16 67%
Nursing and Health Professions 1 4%
Environmental Science 1 4%
Social Sciences 1 4%
Chemistry 1 4%
Other 0 0%
Unknown 4 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 February 2012.
All research outputs
#18,304,230
of 22,662,201 outputs
Outputs from Genetics and Molecular Research
#722
of 1,790 outputs
Outputs of similar age
#195,924
of 244,049 outputs
Outputs of similar age from Genetics and Molecular Research
#45
of 103 outputs
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So far Altmetric has tracked 1,790 research outputs from this source. They receive a mean Attention Score of 1.2. This one is in the 35th percentile – i.e., 35% of its peers scored the same or lower than it.
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We're also able to compare this research output to 103 others from the same source and published within six weeks on either side of this one. This one is in the 6th percentile – i.e., 6% of its contemporaries scored the same or lower than it.