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Royal Society of Chemistry

Body-on-a-chip simulation with gastrointestinal tract and liver tissues suggests that ingested nanoparticles have the potential to cause liver injury

Overview of attention for article published in Lab on a Chip - Miniaturisation for Chemistry & Biology, January 2014
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (98th percentile)
  • High Attention Score compared to outputs of the same age and source (98th percentile)

Mentioned by

news
9 news outlets
blogs
1 blog
twitter
5 X users
patent
3 patents
weibo
1 weibo user
facebook
2 Facebook pages
googleplus
1 Google+ user

Citations

dimensions_citation
220 Dimensions

Readers on

mendeley
258 Mendeley
Title
Body-on-a-chip simulation with gastrointestinal tract and liver tissues suggests that ingested nanoparticles have the potential to cause liver injury
Published in
Lab on a Chip - Miniaturisation for Chemistry & Biology, January 2014
DOI 10.1039/c4lc00371c
Pubmed ID
Authors

Mandy B. Esch, Gretchen J. Mahler, Tracy Stokol, Michael L. Shuler

Abstract

The use of nanoparticles in medical applications is highly anticipated, and at the same time little is known about how these nanoparticles affect human tissues. Here we have simulated the oral uptake of 50 nm carboxylated polystyrene nanoparticles with a microscale body-on-a-chip system (also referred to as multi-tissue microphysiological system or micro Cell Culture Analog). Using the 'GI tract-liver-other tissues' system allowed us to observe compounding effects and detect liver tissue injury at lower nanoparticle concentrations than was expected from experiments with single tissues. To construct this system, we combined in vitro models of the human intestinal epithelium, represented by a co-culture of enterocytes (Caco-2) and mucin-producing cells (TH29-MTX), and the liver, represented by HepG2/C3A cells, within one microfluidic device. The device also contained chambers that together represented the liquid portions of all other organs of the human body. Measuring the transport of 50 nm carboxylated polystyrene nanoparticles across the Caco-2/HT29-MTX co-culture, we found that this multi-cell layer presents an effective barrier to 90.5 ± 2.9% of the nanoparticles. Further, our simulation suggests that a larger fraction of the 9.5 ± 2.9% nanoparticles that travelled across the Caco-2/HT29-MTX cell layer were not large nanoparticle aggregates, but primarily single nanoparticles and small aggregates. After crossing the GI tract epithelium, nanoparticles that were administered in high doses estimated in terms of possible daily human consumption (240 and 480 × 10(11) nanoparticles mL(-1)) induced the release of aspartate aminotransferase (AST), an intracellular enzyme of the liver that indicates liver cell injury. Our results indicate that body-on-a-chip devices are highly relevant in vitro models for evaluating nanoparticle interactions with human tissues.

X Demographics

X Demographics

The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 258 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 3 1%
France 2 <1%
Germany 1 <1%
Brazil 1 <1%
Italy 1 <1%
Unknown 250 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 69 27%
Student > Master 36 14%
Researcher 32 12%
Student > Bachelor 18 7%
Student > Doctoral Student 15 6%
Other 35 14%
Unknown 53 21%
Readers by discipline Count As %
Engineering 53 21%
Agricultural and Biological Sciences 42 16%
Biochemistry, Genetics and Molecular Biology 26 10%
Pharmacology, Toxicology and Pharmaceutical Science 14 5%
Medicine and Dentistry 14 5%
Other 37 14%
Unknown 72 28%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 80. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 September 2023.
All research outputs
#536,323
of 25,457,858 outputs
Outputs from Lab on a Chip - Miniaturisation for Chemistry & Biology
#81
of 5,941 outputs
Outputs of similar age
#5,324
of 319,666 outputs
Outputs of similar age from Lab on a Chip - Miniaturisation for Chemistry & Biology
#5
of 358 outputs
Altmetric has tracked 25,457,858 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 5,941 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 7.4. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 319,666 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 98% of its contemporaries.
We're also able to compare this research output to 358 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 98% of its contemporaries.