| Title |
Direct conversion of quiescent cardiomyocytes to pacemaker cells by expression of Tbx18
|
|---|---|
| Published in |
Nature Biotechnology, December 2012
|
| DOI | 10.1038/nbt.2465 |
| Pubmed ID | |
| Authors |
Nidhi Kapoor, Wenbin Liang, Eduardo Marbán, Hee Cheol Cho |
| Abstract |
The heartbeat originates within the sinoatrial node (SAN), a small structure containing <10,000 genuine pacemaker cells. If the SAN fails, the ∼5 billion working cardiomyocytes downstream of it become quiescent, leading to circulatory collapse in the absence of electronic pacemaker therapy. Here we demonstrate conversion of rodent cardiomyocytes to SAN cells in vitro and in vivo by expression of Tbx18, a gene critical for early SAN specification. Within days of in vivo Tbx18 transduction, 9.2% of transduced, ventricular cardiomyocytes develop spontaneous electrical firing physiologically indistinguishable from that of SAN cells, along with morphological and epigenetic features characteristic of SAN cells. In vivo, focal Tbx18 gene transfer in the guinea-pig ventricle yields ectopic pacemaker activity, correcting a bradycardic disease phenotype. Myocytes transduced in vivo acquire the cardinal tapering morphology and physiological automaticity of native SAN pacemaker cells. The creation of induced SAN pacemaker (iSAN) cells opens new prospects for bioengineered pacemakers. |
X Demographics
The data shown below were collected from the profiles of 36 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 12 | 33% |
| United Kingdom | 3 | 8% |
| Japan | 2 | 6% |
| India | 1 | 3% |
| Italy | 1 | 3% |
| Venezuela, Bolivarian Republic of | 1 | 3% |
| Peru | 1 | 3% |
| Canada | 1 | 3% |
| Dominican Republic | 1 | 3% |
| Other | 4 | 11% |
| Unknown | 9 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 26 | 72% |
| Scientists | 7 | 19% |
| Science communicators (journalists, bloggers, editors) | 2 | 6% |
| Practitioners (doctors, other healthcare professionals) | 1 | 3% |
Mendeley readers
The data shown below were compiled from readership statistics for 284 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | 1% |
| Portugal | 1 | <1% |
| France | 1 | <1% |
| United Kingdom | 1 | <1% |
| Canada | 1 | <1% |
| Netherlands | 1 | <1% |
| China | 1 | <1% |
| Iran, Islamic Republic of | 1 | <1% |
| Spain | 1 | <1% |
| Other | 1 | <1% |
| Unknown | 271 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 67 | 24% |
| Researcher | 48 | 17% |
| Student > Bachelor | 40 | 14% |
| Student > Master | 31 | 11% |
| Student > Doctoral Student | 18 | 6% |
| Other | 50 | 18% |
| Unknown | 30 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 89 | 31% |
| Medicine and Dentistry | 55 | 19% |
| Biochemistry, Genetics and Molecular Biology | 48 | 17% |
| Engineering | 26 | 9% |
| Neuroscience | 5 | 2% |
| Other | 21 | 7% |
| Unknown | 40 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 223. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 May 2023.
All research outputs
#155,073
of 23,926,844 outputs
Outputs from Nature Biotechnology
#317
of 8,449 outputs
Outputs of similar age
#1,005
of 284,694 outputs
Outputs of similar age from Nature Biotechnology
#2
of 114 outputs
Altmetric has tracked 23,926,844 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,449 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 42.3. This one has done particularly well, scoring higher than 96% of its peers.
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We're also able to compare this research output to 114 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.