| Title |
Quantitative visualization of DNA G-quadruplex structures in human cells
|
|---|---|
| Published in |
Nature Chemistry, January 2013
|
| DOI | 10.1038/nchem.1548 |
| Pubmed ID | |
| Authors |
Giulia Biffi, David Tannahill, John McCafferty, Shankar Balasubramanian |
| Abstract |
Four-stranded G-quadruplex nucleic acid structures are of great interest as their high thermodynamic stability under near-physiological conditions suggests that they could form in cells. Here we report the generation and application of an engineered, structure-specific antibody employed to quantitatively visualize DNA G-quadruplex structures in human cells. We show explicitly that G-quadruplex formation in DNA is modulated during cell-cycle progression and that endogenous G-quadruplex DNA structures can be stabilized by a small-molecule ligand. Together these findings provide substantive evidence for the formation of G-quadruplex structures in the genome of mammalian cells and corroborate the application of stabilizing ligands in a cellular context to target G-quadruplexes and intervene with their function. |
X Demographics
The data shown below were collected from the profiles of 209 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 35 | 17% |
| United Kingdom | 24 | 11% |
| Spain | 9 | 4% |
| Italy | 6 | 3% |
| Canada | 6 | 3% |
| Chile | 4 | 2% |
| Mexico | 4 | 2% |
| Netherlands | 4 | 2% |
| Argentina | 3 | 1% |
| Other | 31 | 15% |
| Unknown | 83 | 40% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 153 | 73% |
| Scientists | 46 | 22% |
| Practitioners (doctors, other healthcare professionals) | 6 | 3% |
| Science communicators (journalists, bloggers, editors) | 4 | 2% |
Mendeley readers
The data shown below were compiled from readership statistics for 1,432 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 20 | 1% |
| United Kingdom | 16 | 1% |
| France | 7 | <1% |
| Brazil | 7 | <1% |
| Germany | 5 | <1% |
| Netherlands | 4 | <1% |
| Italy | 4 | <1% |
| Japan | 4 | <1% |
| Canada | 4 | <1% |
| Other | 20 | 1% |
| Unknown | 1341 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 418 | 29% |
| Researcher | 217 | 15% |
| Student > Master | 180 | 13% |
| Student > Bachelor | 152 | 11% |
| Student > Doctoral Student | 60 | 4% |
| Other | 186 | 13% |
| Unknown | 219 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 385 | 27% |
| Biochemistry, Genetics and Molecular Biology | 329 | 23% |
| Chemistry | 283 | 20% |
| Medicine and Dentistry | 42 | 3% |
| Physics and Astronomy | 35 | 2% |
| Other | 106 | 7% |
| Unknown | 252 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 586. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 October 2023.
All research outputs
#40,431
of 25,773,273 outputs
Outputs from Nature Chemistry
#12
of 3,388 outputs
Outputs of similar age
#183
of 295,802 outputs
Outputs of similar age from Nature Chemistry
#1
of 46 outputs
Altmetric has tracked 25,773,273 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,388 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 36.1. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 295,802 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 46 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 97% of its contemporaries.