| Title |
Crystal structure of the 14-subunit RNA polymerase I
|
|---|---|
| Published in |
Nature, October 2013
|
| DOI | 10.1038/nature12636 |
| Pubmed ID | |
| Authors |
Carlos Fernández-Tornero, María Moreno-Morcillo, Umar J. Rashid, Nicholas M. I. Taylor, Federico M. Ruiz, Tim Gruene, Pierre Legrand, Ulrich Steuerwald, Christoph W. Müller |
| Abstract |
Protein biosynthesis depends on the availability of ribosomes, which in turn relies on ribosomal RNA production. In eukaryotes, this process is carried out by RNA polymerase I (Pol I), a 14-subunit enzyme, the activity of which is a major determinant of cell growth. Here we present the crystal structure of Pol I from Saccharomyces cerevisiae at 3.0 Å resolution. The Pol I structure shows a compact core with a wide DNA-binding cleft and a tightly anchored stalk. An extended loop mimics the DNA backbone in the cleft and may be involved in regulating Pol I transcription. Subunit A12.2 extends from the A190 jaw to the active site and inserts a transcription elongation factor TFIIS-like zinc ribbon into the nucleotide triphosphate entry pore, providing insight into the role of A12.2 in RNA cleavage and Pol I insensitivity to α-amanitin. The A49-A34.5 heterodimer embraces subunit A135 through extended arms, thereby contacting and potentially regulating subunit A12.2. |
X Demographics
The data shown below were collected from the profiles of 59 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Mexico | 5 | 8% |
| United Kingdom | 5 | 8% |
| Spain | 4 | 7% |
| Argentina | 4 | 7% |
| United States | 4 | 7% |
| Venezuela, Bolivarian Republic of | 3 | 5% |
| Norway | 2 | 3% |
| Canada | 2 | 3% |
| France | 2 | 3% |
| Other | 5 | 8% |
| Unknown | 23 | 39% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 47 | 80% |
| Scientists | 9 | 15% |
| Practitioners (doctors, other healthcare professionals) | 2 | 3% |
| Science communicators (journalists, bloggers, editors) | 1 | 2% |
Mendeley readers
The data shown below were compiled from readership statistics for 223 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | <1% |
| Netherlands | 1 | <1% |
| France | 1 | <1% |
| United Kingdom | 1 | <1% |
| Taiwan | 1 | <1% |
| United States | 1 | <1% |
| Unknown | 217 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 52 | 23% |
| Researcher | 51 | 23% |
| Student > Bachelor | 27 | 12% |
| Student > Master | 17 | 8% |
| Student > Doctoral Student | 13 | 6% |
| Other | 32 | 14% |
| Unknown | 31 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 84 | 38% |
| Biochemistry, Genetics and Molecular Biology | 70 | 31% |
| Chemistry | 10 | 4% |
| Medicine and Dentistry | 7 | 3% |
| Immunology and Microbiology | 5 | 2% |
| Other | 12 | 5% |
| Unknown | 35 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 101. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 September 2019.
All research outputs
#425,491
of 25,837,817 outputs
Outputs from Nature
#20,520
of 98,779 outputs
Outputs of similar age
#3,308
of 227,058 outputs
Outputs of similar age from Nature
#303
of 1,070 outputs
Altmetric has tracked 25,837,817 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 98th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 98,779 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 102.5. This one has done well, scoring higher than 79% of its peers.
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We're also able to compare this research output to 1,070 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.