| Title |
Structure of a mammalian ryanodine receptor
|
|---|---|
| Published in |
Nature, December 2014
|
| DOI | 10.1038/nature13950 |
| Pubmed ID | |
| Authors |
Ran Zalk, Oliver B. Clarke, Amédée des Georges, Robert A. Grassucci, Steven Reiken, Filippo Mancia, Wayne A. Hendrickson, Joachim Frank, Andrew R. Marks |
| Abstract |
Ryanodine receptors (RyRs) mediate the rapid release of calcium (Ca(2+)) from intracellular stores into the cytosol, which is essential for numerous cellular functions including excitation-contraction coupling in muscle. Lack of sufficient structural detail has impeded understanding of RyR gating and regulation. Here we report the closed-state structure of the 2.3-megadalton complex of the rabbit skeletal muscle type 1 RyR (RyR1), solved by single-particle electron cryomicroscopy at an overall resolution of 4.8 Å. We fitted a polyalanine-level model to all 3,757 ordered residues in each protomer, defining the transmembrane pore in unprecedented detail and placing all cytosolic domains as tertiary folds. The cytosolic assembly is built on an extended α-solenoid scaffold connecting key regulatory domains to the pore. The RyR1 pore architecture places it in the six-transmembrane ion channel superfamily. A unique domain inserted between the second and third transmembrane helices interacts intimately with paired EF-hands originating from the α-solenoid scaffold, suggesting a mechanism for channel gating by Ca(2+). |
X Demographics
The data shown below were collected from the profiles of 11 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 27% |
| New Zealand | 1 | 9% |
| Germany | 1 | 9% |
| Unknown | 6 | 55% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 9 | 82% |
| Scientists | 1 | 9% |
| Science communicators (journalists, bloggers, editors) | 1 | 9% |
Mendeley readers
The data shown below were compiled from readership statistics for 384 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 4 | 1% |
| United States | 4 | 1% |
| France | 2 | <1% |
| Japan | 2 | <1% |
| Ireland | 1 | <1% |
| Switzerland | 1 | <1% |
| Unknown | 370 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 94 | 24% |
| Researcher | 68 | 18% |
| Student > Master | 51 | 13% |
| Student > Bachelor | 38 | 10% |
| Professor | 17 | 4% |
| Other | 61 | 16% |
| Unknown | 55 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 116 | 30% |
| Biochemistry, Genetics and Molecular Biology | 99 | 26% |
| Medicine and Dentistry | 32 | 8% |
| Chemistry | 14 | 4% |
| Physics and Astronomy | 11 | 3% |
| Other | 44 | 11% |
| Unknown | 68 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 72. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 February 2020.
All research outputs
#587,658
of 25,165,468 outputs
Outputs from Nature
#24,594
of 96,870 outputs
Outputs of similar age
#7,179
of 374,336 outputs
Outputs of similar age from Nature
#413
of 956 outputs
Altmetric has tracked 25,165,468 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 96,870 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 102.4. This one has gotten more attention than average, scoring higher than 74% of its peers.
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