Title |
RGB marking facilitates multicolor clonal cell tracking
|
---|---|
Published in |
Nature Medicine, March 2011
|
DOI | 10.1038/nm.2338 |
Pubmed ID | |
Authors |
Kristoffer Weber, Michael Thomaschewski, Michael Warlich, Tassilo Volz, Kerstin Cornils, Birte Niebuhr, Maike Täger, Marc Lütgehetmann, Jörg-Matthias Pollok, Carol Stocking, Maura Dandri, Daniel Benten, Boris Fehse |
Abstract |
We simultaneously transduced cells with three lentiviral gene ontology (LeGO) vectors encoding red, green or blue fluorescent proteins. Individual cells were thereby marked by different combinations of inserted vectors, resulting in the generation of numerous mixed colors, a principle we named red-green-blue (RGB) marking. We show that lentiviral vector-mediated RGB marking remained stable after cell division, thus facilitating the analysis of clonal cell fates in vitro and in vivo. Particularly, we provide evidence that RGB marking allows assessment of clonality after regeneration of injured livers by transplanted primary hepatocytes. We also used RGB vectors to mark hematopoietic stem/progenitor cells that generated colored spleen colonies. Finally, based on limiting-dilution and serial transplantation assays with tumor cells, we found that clonal tumor cells retained their specific color-code over extensive periods of time. We conclude that RGB marking represents a useful tool for cell clonality studies in tissue regeneration and pathology. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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France | 5 | 2% |
Germany | 4 | 1% |
United States | 3 | 1% |
United Kingdom | 2 | <1% |
Italy | 1 | <1% |
Spain | 1 | <1% |
Portugal | 1 | <1% |
Unknown | 278 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 84 | 28% |
Student > Ph. D. Student | 77 | 26% |
Student > Master | 21 | 7% |
Student > Bachelor | 21 | 7% |
Professor > Associate Professor | 19 | 6% |
Other | 45 | 15% |
Unknown | 28 | 9% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 137 | 46% |
Biochemistry, Genetics and Molecular Biology | 46 | 16% |
Medicine and Dentistry | 32 | 11% |
Neuroscience | 12 | 4% |
Physics and Astronomy | 8 | 3% |
Other | 26 | 9% |
Unknown | 34 | 12% |