| Title |
Crystal structure of an orthologue of the NaChBac voltage-gated sodium channel
|
|---|---|
| Published in |
Nature, May 2012
|
| DOI | 10.1038/nature11054 |
| Pubmed ID | |
| Authors |
Xu Zhang, Wenlin Ren, Paul DeCaen, Chuangye Yan, Xiao Tao, Lin Tang, Jingjing Wang, Kazuya Hasegawa, Takashi Kumasaka, Jianhua He, Jiawei Wang, David E. Clapham, Nieng Yan |
| Abstract |
Voltage-gated sodium (Na(v)) channels are essential for the rapid depolarization of nerve and muscle, and are important drug targets. Determination of the structures of Na(v) channels will shed light on ion channel mechanisms and facilitate potential clinical applications. A family of bacterial Na(v) channels, exemplified by the Na(+)-selective channel of bacteria (NaChBac), provides a useful model system for structure-function analysis. Here we report the crystal structure of Na(v)Rh, a NaChBac orthologue from the marine alphaproteobacterium HIMB114 (Rickettsiales sp. HIMB114; denoted Rh), at 3.05 Å resolution. The channel comprises an asymmetric tetramer. The carbonyl oxygen atoms of Thr 178 and Leu 179 constitute an inner site within the selectivity filter where a hydrated Ca(2+) resides in the crystal structure. The outer mouth of the Na(+) selectivity filter, defined by Ser 181 and Glu 183, is closed, as is the activation gate at the intracellular side of the pore. The voltage sensors adopt a depolarized conformation in which all the gating charges are exposed to the extracellular environment. We propose that Na(v)Rh is in an 'inactivated' conformation. Comparison of Na(v)Rh with Na(v)Ab reveals considerable conformational rearrangements that may underlie the electromechanical coupling mechanism of voltage-gated channels. |
X Demographics
The data shown below were collected from the profiles of 12 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 4 | 33% |
| Japan | 1 | 8% |
| Canada | 1 | 8% |
| United States | 1 | 8% |
| Unknown | 5 | 42% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 10 | 83% |
| Scientists | 2 | 17% |
Mendeley readers
The data shown below were compiled from readership statistics for 386 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 7 | 2% |
| United Kingdom | 6 | 2% |
| Germany | 3 | <1% |
| Japan | 2 | <1% |
| China | 2 | <1% |
| Mexico | 1 | <1% |
| Argentina | 1 | <1% |
| Belgium | 1 | <1% |
| Brazil | 1 | <1% |
| Other | 4 | 1% |
| Unknown | 358 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 96 | 25% |
| Researcher | 84 | 22% |
| Student > Master | 51 | 13% |
| Student > Bachelor | 31 | 8% |
| Professor | 21 | 5% |
| Other | 63 | 16% |
| Unknown | 40 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 150 | 39% |
| Biochemistry, Genetics and Molecular Biology | 77 | 20% |
| Chemistry | 37 | 10% |
| Medicine and Dentistry | 21 | 5% |
| Neuroscience | 16 | 4% |
| Other | 41 | 11% |
| Unknown | 44 | 11% |
Attention Score in Context
This research output has an Altmetric Attention Score of 18. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 June 2016.
All research outputs
#1,752,549
of 22,665,794 outputs
Outputs from Nature
#38,989
of 90,603 outputs
Outputs of similar age
#11,229
of 163,617 outputs
Outputs of similar age from Nature
#572
of 979 outputs
Altmetric has tracked 22,665,794 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 92nd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 90,603 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 99.2. This one has gotten more attention than average, scoring higher than 56% of its peers.
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