| Title |
Key stabilizing elements of protein structure identified through pressure and temperature perturbation of its hydrogen bond network
|
|---|---|
| Published in |
Nature Chemistry, July 2012
|
| DOI | 10.1038/nchem.1396 |
| Pubmed ID | |
| Authors |
Lydia Nisius, Stephan Grzesiek |
| Abstract |
Hydrogen bonds are key constituents of biomolecular structures, and their response to external perturbations may reveal important insights about the most stable components of a structure. NMR spectroscopy can probe hydrogen bond deformations at very high resolution through hydrogen bond scalar couplings (HBCs). However, the small size of HBCs has so far prevented a comprehensive quantitative characterization of protein hydrogen bonds as a function of the basic thermodynamic parameters of pressure and temperature. Using a newly developed pressure cell, we have now mapped pressure- and temperature-dependent changes of 31 hydrogen bonds in ubiquitin by measuring HBCs with very high precision. Short-range hydrogen bonds are only moderately perturbed, but many hydrogen bonds with large sequence separations (high contact order) show greater changes. In contrast, other high-contact-order hydrogen bonds remain virtually unaffected. The specific stabilization of such topologically important connections may present a general principle with which to achieve protein stability and to preserve structural integrity during protein function. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 50% |
| Japan | 1 | 25% |
| United States | 1 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 50% |
| Scientists | 1 | 25% |
| Science communicators (journalists, bloggers, editors) | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 113 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Poland | 2 | 2% |
| United States | 2 | 2% |
| Vietnam | 1 | <1% |
| Sweden | 1 | <1% |
| Taiwan | 1 | <1% |
| Portugal | 1 | <1% |
| United Kingdom | 1 | <1% |
| China | 1 | <1% |
| Unknown | 103 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 39 | 35% |
| Researcher | 22 | 19% |
| Student > Doctoral Student | 8 | 7% |
| Student > Bachelor | 7 | 6% |
| Professor > Associate Professor | 6 | 5% |
| Other | 20 | 18% |
| Unknown | 11 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 37 | 33% |
| Chemistry | 32 | 28% |
| Biochemistry, Genetics and Molecular Biology | 18 | 16% |
| Physics and Astronomy | 8 | 7% |
| Engineering | 2 | 2% |
| Other | 4 | 4% |
| Unknown | 12 | 11% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 October 2014.
All research outputs
#4,571,410
of 22,669,724 outputs
Outputs from Nature Chemistry
#2,119
of 2,992 outputs
Outputs of similar age
#31,928
of 164,716 outputs
Outputs of similar age from Nature Chemistry
#36
of 48 outputs
Altmetric has tracked 22,669,724 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,992 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 35.1. This one is in the 29th percentile – i.e., 29% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 164,716 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 48 others from the same source and published within six weeks on either side of this one. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.