↓ Skip to main content

PNAS

RNA-directed gene editing specifically eradicates latent and prevents new HIV-1 infection

Overview of attention for article published in Proceedings of the National Academy of Sciences of the United States of America, July 2014
Altmetric Badge

About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (99th percentile)
  • High Attention Score compared to outputs of the same age and source (98th percentile)

Citations

dimensions_citation
468 Dimensions

Readers on

mendeley
900 Mendeley
citeulike
3 CiteULike
Title
RNA-directed gene editing specifically eradicates latent and prevents new HIV-1 infection
Published in
Proceedings of the National Academy of Sciences of the United States of America, July 2014
DOI 10.1073/pnas.1405186111
Pubmed ID
Authors

Wenhui Hu, Rafal Kaminski, Fan Yang, Yonggang Zhang, Laura Cosentino, Fang Li, Biao Luo, David Alvarez-Carbonell, Yoelvis Garcia-Mesa, Jonathan Karn, Xianming Mo, Kamel Khalili

Abstract

AIDS remains incurable due to the permanent integration of HIV-1 into the host genome, imparting risk of viral reactivation even after antiretroviral therapy. New strategies are needed to ablate the viral genome from latently infected cells, because current methods are too inefficient and prone to adverse off-target effects. To eliminate the integrated HIV-1 genome, we used the Cas9/guide RNA (gRNA) system, in single and multiplex configurations. We identified highly specific targets within the HIV-1 LTR U3 region that were efficiently edited by Cas9/gRNA, inactivating viral gene expression and replication in latently infected microglial, promonocytic, and T cells. Cas9/gRNAs caused neither genotoxicity nor off-target editing to the host cells, and completely excised a 9,709-bp fragment of integrated proviral DNA that spanned from its 5' to 3' LTRs. Furthermore, the presence of multiplex gRNAs within Cas9-expressing cells prevented HIV-1 infection. Our results suggest that Cas9/gRNA can be engineered to provide a specific, efficacious prophylactic and therapeutic approach against AIDS.

X Demographics

X Demographics

The data shown below were collected from the profiles of 222 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 900 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 23 3%
Germany 5 <1%
United Kingdom 4 <1%
Japan 4 <1%
Canada 3 <1%
France 2 <1%
South Africa 2 <1%
Spain 2 <1%
Mexico 2 <1%
Other 7 <1%
Unknown 846 94%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 184 20%
Student > Ph. D. Student 178 20%
Student > Master 138 15%
Researcher 127 14%
Student > Doctoral Student 32 4%
Other 104 12%
Unknown 137 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 316 35%
Biochemistry, Genetics and Molecular Biology 200 22%
Medicine and Dentistry 71 8%
Immunology and Microbiology 59 7%
Chemistry 20 2%
Other 74 8%
Unknown 160 18%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 807. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 February 2024.
All research outputs
#23,368
of 25,528,120 outputs
Outputs from Proceedings of the National Academy of Sciences of the United States of America
#715
of 103,299 outputs
Outputs of similar age
#130
of 239,913 outputs
Outputs of similar age from Proceedings of the National Academy of Sciences of the United States of America
#11
of 916 outputs
Altmetric has tracked 25,528,120 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 103,299 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 39.5. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 239,913 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 916 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 98% of its contemporaries.