Genome-wide mapping of methylated adenine residues in pathogenic Escherichia coli using single-molecule real-time sequencing

Overview of attention for article published in Nature Biotechnology, November 2012
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  • In the top 5% of all articles scored by Altmetric
  • High score compared to articles of the same age (98th percentile)
  • High score compared to articles of the same age and source (90th percentile)

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713 Mendeley
Article title
Genome-wide mapping of methylated adenine residues in pathogenic Escherichia coli using single-molecule real-time sequencing
Published in
Nature Biotechnology, November 2012
DOI 10.1038/nbt.2432
Pubmed ID
Authors

Gang Fang, Diana Munera, David I Friedman, Anjali Mandlik, Michael C Chao, Onureena Banerjee, Zhixing Feng, Bojan Losic, Milind C Mahajan, Omar J Jabado, Gintaras Deikus, Tyson A Clark, Khai Luong, Iain A Murray, Brigid M Davis, Alona Keren-Paz, Andrew Chess, Richard J Roberts, Jonas Korlach, Steve W Turner, Vipin Kumar, Matthew K Waldor, Eric E Schadt

Abstract

Single-molecule real-time (SMRT) DNA sequencing allows the systematic detection of chemical modifications such as methylation but has not previously been applied on a genome-wide scale. We used this approach to detect 49,311 putative 6-methyladenine (m6A) residues and 1,407 putative 5-methylcytosine (m5C) residues in the genome of a pathogenic Escherichia coli strain. We obtained strand-specific information for methylation sites and a quantitative assessment of the frequency of methylation at each modified position. We deduced the sequence motifs recognized by the methyltransferase enzymes present in this strain without prior knowledge of their specificity. Furthermore, we found that deletion of a phage-encoded methyltransferase-endonuclease (restriction-modification; RM) system induced global transcriptional changes and led to gene amplification, suggesting that the role of RM systems extends beyond protecting host genomes from foreign DNA.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 713 Mendeley readers of this article. Click here to see the article's page on the Mendeley website.

Geographical breakdown

Country Count As %
United States 18 3%
United Kingdom 4 1%
Germany 3 0%
Belgium 3 0%
Japan 2 0%
Brazil 2 0%
Sweden 1 0%
China 1 0%
Norway 1 0%
Other 6 1%
Unknown 672 94%

Demographic breakdown

Readers by professional status Count As %
Ph.D. Student 50 7%
Post Doc 25 4%
Student (Master) 21 3%
Researcher (at a non-Academic Institution) 10 1%
Researcher (at an Academic Institution) 10 1%
Other 39 5%
Unknown 558 78%
Readers by discipline Count As %
Biological Sciences 136 19%
Computer and Information Science 6 1%
Medicine 5 1%
Engineering 4 1%
Chemistry 3 0%
Other 1 0%
Unknown 558 78%

Score in context

This article has an Altmetric score of 47. This is our high-level measure of the quality and quantity of online attention that this article has received. This score was calculated when the article was last mentioned on 07 July 2015.
All articles
#63,707
of 4,123,067 articles
Articles in Nature Biotechnology
#201
of 2,922 articles
Articles of similar age
#1,409
of 79,895 articles
Articles of similar age in Nature Biotechnology
#10
of 108 articles
Altmetric has tracked 4,123,067 articles across all sources so far. Compared to these this article has done particularly well and is in the 98th percentile: it's in the top 5% of all articles ever tracked by Altmetric.
So far Altmetric has tracked 2,922 articles from this source. They typically receive a lot more attention than average, with a mean score of 14.5. This article has done particularly well, scoring higher than 93% of its peers.
Older articles will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this score to the 79,895 tracked articles that were published within six weeks on either side of this one in any source. This article has done particularly well, scoring higher than 98% of its contemporaries.
We're also able to compare this article to 108 articles from the same source and published within six weeks on either side of this one. This article has done particularly well, scoring higher than 90% of its contemporaries.