Title |
AMP-activated Protein Kinase Activation by 5-Aminoimidazole-4-carbox-amide-1-β-d-ribofuranoside (AICAR) Reduces Lipoteichoic Acid-induced Lung Inflammation
|
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Published in |
Journal of Biological Chemistry, January 2013
|
DOI | 10.1074/jbc.m112.413138 |
Pubmed ID | |
Authors |
Arie J. Hoogendijk, Sandra S. Pinhanços, Tom van der Poll, Catharina W. Wieland |
Abstract |
Adenosine monophosphate-activated protein (AMP)-activated kinase (AMPK) is a highly conserved kinase that plays a key role in energy homeostasis. Activation of AMPK was shown to reduce inflammation in response to lipolysaccharide in vitro and in vivo. 5-Aminoimidazole-4-carbox-amide-1-β-D-ribofuranoside (AICAR) is intracellularly converted to the AMP analog ZMP, which activates AMPK. Lipoteichoic acid (LTA) is a major component of the cell wall of Gram-positive bacteria that can trigger inflammatory responses. In contrast to lipopolysaccharide, little is known on the effects of AMPK activation in LTA-triggered innate immune responses. Here, we studied the potency of AMPK activation to reduce LTA-induced inflammation in vitro and in lungs in vivo. Activation of AMPK in vitro reduced cytokine production in the alveolar macrophage cell line MH-S. In vivo, AMPK activation reduced LTA-induced neutrophil influx, as well as protein leak and cytokine/chemokine levels in the bronchoalveolar space. In conclusion, AMPK activation inhibits LTA-induced lung inflammation in mice. |
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Russia | 1 | 4% |
France | 1 | 4% |
Germany | 1 | 4% |
Unknown | 21 | 84% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 4 | 16% |
Researcher | 4 | 16% |
Professor > Associate Professor | 3 | 12% |
Student > Master | 3 | 12% |
Student > Ph. D. Student | 3 | 12% |
Other | 3 | 12% |
Unknown | 5 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 12 | 48% |
Biochemistry, Genetics and Molecular Biology | 4 | 16% |
Immunology and Microbiology | 2 | 8% |
Medicine and Dentistry | 2 | 8% |
Unknown | 5 | 20% |