Hepatitis C Virus Pathogen Associated Molecular Pattern (PAMP) Triggers Production of Lambda-Interferons by Human Plasmacytoid Dendritic Cells

Overview of attention for article published in PLoS Pathogens, January 2013
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Article title
Hepatitis C Virus Pathogen Associated Molecular Pattern (PAMP) Triggers Production of Lambda-Interferons by Human Plasmacytoid Dendritic Cells
Published in
PLoS Pathogens, January 2013
DOI 10.1371/journal.ppat.1003316
Pubmed ID
Authors

Amy E. L. Stone

Abstract

Plasmacytoid Dendritic Cells (pDCs) represent a key immune cell in the defense against viruses. Through pattern recognition receptors (PRRs), these cells detect viral pathogen associated molecular patterns (PAMPs) and initiate an Interferon (IFN) response. pDCs produce the antiviral IFNs including the well-studied Type I and the more recently described Type III. Recent genome wide association studies (GWAS) have implicated Type III IFNs in HCV clearance. We examined the IFN response induced in a pDC cell line and ex vivo human pDCs by a region of the HCV genome referred to as the HCV PAMP. This RNA has been shown previously to be immunogenic in hepatocytes, whereas the conserved X-region RNA is not. We show that in response to the HCV PAMP, pDC-GEN2.2 cells upregulate and secrete Type III (in addition to Type I) IFNs and upregulate PRR genes and proteins. We also demonstrate that the recognition of this RNA is dependent on RIG-I-like Receptors (RLRs) and Toll-like Receptors (TLRs), challenging the dogma that RLRs are dispensable in pDCs. The IFNs produced by these cells in response to the HCV PAMP also control HCV replication in vitro. These data are recapitulated in ex vivo pDCs isolated from healthy donors. Together, our data shows that pDCs respond robustly to HCV RNA to make Type III Interferons that control viral replication. This may represent a novel therapeutic strategy for the treatment of HCV.

Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this article. Click here to see the article's page on the Mendeley website.

Geographical breakdown

Country Count As %
Japan 1 3%
Australia 1 3%
Canada 1 3%
United States 1 3%
Unknown 25 86%

Demographic breakdown

Readers by professional status Count As %
Post Doc 8 28%
Ph.D. Student 7 24%
Researcher (at an Academic Institution) 5 17%
Student (Master) 3 10%
Doctoral Student 2 7%
Other 4 14%
Readers by discipline Count As %
Biological Sciences 24 83%
Medicine 5 17%

Score in context

This article has an Altmetric score of 0.25. This is our high-level measure of the quality and quantity of online attention that this article has received. This score was calculated when the article was last mentioned on 19 April 2013.
All articles
#2,897,991
of 3,632,750 articles
Articles in PLoS Pathogens
#2,610
of 2,930 articles
Articles of similar age
#65,738
of 85,036 articles
Articles of similar age in PLoS Pathogens
#122
of 144 articles
Altmetric has tracked 3,632,750 articles across all sources so far. This article is in the 2nd percentile – i.e., 2% of other articles scored the same or lower than it.
So far Altmetric has tracked 2,930 articles from this source. They typically receive more attention than average, with a mean score of 7.9. This article is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older articles will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this score to the 85,036 tracked articles that were published within six weeks on either side of this one in any source. This article is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this article to 144 articles from the same source and published within six weeks on either side of this one. This article is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.