Title |
Structure of RSV Fusion Glycoprotein Trimer Bound to a Prefusion-Specific Neutralizing Antibody
|
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Published in |
Science, April 2013
|
DOI | 10.1126/science.1234914 |
Pubmed ID | |
Authors |
Jason S. McLellan, Man Chen, Sherman Leung, Kevin W. Graepel, Xiulian Du, Yongping Yang, Tongqing Zhou, Ulrich Baxa, Etsuko Yasuda, Tim Beaumont, Azad Kumar, Kayvon Modjarrad, Zizheng Zheng, Min Zhao, Ningshao Xia, Peter D. Kwong, Barney S. Graham |
Abstract |
The prefusion state of respiratory syncytial virus (RSV) fusion (F) glycoprotein is the target of most RSV-neutralizing activity in human sera, but its metastability has hindered characterization. To overcome this obstacle, we identified prefusion-specific antibodies that were substantially more potent than the prophylactic antibody palivizumab. The cocrystal structure for one of these antibodies, D25, in complex with the F glycoprotein revealed D25 to lock F in its prefusion state by binding to a quaternary epitope at the trimer apex. Electron microscopy showed that two other antibodies, AM22 and 5C4, also bound to the newly identified site of vulnerability, which we named antigenic site Ø. These studies should enable design of improved vaccine antigens and define new targets for passive prevention of RSV-induced disease. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Japan | 9 | 21% |
United States | 5 | 12% |
United Kingdom | 5 | 12% |
Venezuela, Bolivarian Republic of | 1 | 2% |
India | 1 | 2% |
Sweden | 1 | 2% |
Saint Barthélemy | 1 | 2% |
Ireland | 1 | 2% |
Canada | 1 | 2% |
Other | 2 | 5% |
Unknown | 16 | 37% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 33 | 77% |
Scientists | 6 | 14% |
Science communicators (journalists, bloggers, editors) | 3 | 7% |
Practitioners (doctors, other healthcare professionals) | 1 | 2% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | <1% |
France | 1 | <1% |
South Africa | 1 | <1% |
Ireland | 1 | <1% |
Spain | 1 | <1% |
United Kingdom | 1 | <1% |
Unknown | 410 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 97 | 23% |
Researcher | 75 | 18% |
Student > Bachelor | 40 | 10% |
Student > Master | 26 | 6% |
Student > Doctoral Student | 19 | 5% |
Other | 57 | 14% |
Unknown | 103 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 103 | 25% |
Biochemistry, Genetics and Molecular Biology | 81 | 19% |
Immunology and Microbiology | 47 | 11% |
Medicine and Dentistry | 34 | 8% |
Chemistry | 10 | 2% |
Other | 32 | 8% |
Unknown | 110 | 26% |