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DNA barcoding in Mucorales: an inventory of biodiversity

Overview of attention for article published in Persoonia - Molecular Phylogeny and Evolution of Fungi, March 2013
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  • Average Attention Score compared to outputs of the same age and source

Citations

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Title
DNA barcoding in Mucorales: an inventory of biodiversity
Published in
Persoonia - Molecular Phylogeny and Evolution of Fungi, March 2013
DOI 10.3767/003158513x665070
Pubmed ID
Authors

G. Walther, J. Pawłowska, A. Alastruey-Izquierdo, M. Wrzosek, J.L. Rodriguez-Tudela, S. Dolatabadi, A. Chakrabarti, G.S. de Hoog

Abstract

The order Mucorales comprises predominantly fast-growing saprotrophic fungi, some of which are used for the fermentation of foodstuffs but it also includes species known to cause infections in patients with severe immune or metabolic impairments. To inventory biodiversity in Mucorales ITS barcodes of 668 strains in 203 taxa were generated covering more than two thirds of the recognised species. Using the ITS sequences, Molecular Operational Taxonomic Units were defined by a similarity threshold of 99 %. An LSU sequence was generated for each unit as well. Analysis of the LSU sequences revealed that conventional phenotypic classifications of the Mucoraceae are highly artificial. The LSU- and ITS-based trees suggest that characters, such as rhizoids and sporangiola, traditionally used in mucoralean taxonomy are plesiomorphic traits. The ITS region turned out to be an appropriate barcoding marker in Mucorales. It could be sequenced directly in 82 % of the strains and its variability was sufficient to resolve most of the morphospecies. Molecular identification turned out to be problematic only for the species complexes of Mucor circinelloides, M. flavus, M. piriformis and Zygorhynchus moelleri. As many as 12 possibly undescribed species were detected. Intraspecific variability differed widely among mucorealean species ranging from 0 % in Backusella circina to 13.3 % in Cunninghamella echinulata. A high proportion of clinical strains was included for molecular identification. Clinical isolates of Cunninghamella elegans were identified molecularly for the first time. As a result of the phylogenetic analyses several taxonomic and nomenclatural changes became necessary. The genus Backusella was emended to include all species with transitorily recurved sporangiophores. Since this matched molecular data all Mucor species possessing this character were transferred to Backusella. The genus Zygorhynchus was shown to be polyphyletic based on ITS and LSU data. Consequently, Zygorhynchus was abandoned and all species were reclassified in Mucor. Our phylogenetic analyses showed, furthermore, that all non-thermophilic Rhizomucor species belong to Mucor. Accordingly, Rhizomucor endophyticus was transferred to Mucor and Rhizomucor chlamydosporus was synonymised with Mucor indicus. Lecto-, epi- or neotypes were designated for several taxa.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 192 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Iran, Islamic Republic of 1 <1%
United States 1 <1%
Brazil 1 <1%
Unknown 189 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 37 19%
Student > Master 29 15%
Student > Ph. D. Student 26 14%
Student > Doctoral Student 13 7%
Other 10 5%
Other 40 21%
Unknown 37 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 72 38%
Biochemistry, Genetics and Molecular Biology 22 11%
Medicine and Dentistry 14 7%
Immunology and Microbiology 11 6%
Chemistry 5 3%
Other 21 11%
Unknown 47 24%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 February 2021.
All research outputs
#7,363,939
of 25,394,764 outputs
Outputs from Persoonia - Molecular Phylogeny and Evolution of Fungi
#82
of 218 outputs
Outputs of similar age
#58,820
of 207,680 outputs
Outputs of similar age from Persoonia - Molecular Phylogeny and Evolution of Fungi
#4
of 7 outputs
Altmetric has tracked 25,394,764 research outputs across all sources so far. This one has received more attention than most of these and is in the 69th percentile.
So far Altmetric has tracked 218 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.8. This one has gotten more attention than average, scoring higher than 57% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 207,680 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.
We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one. This one has scored higher than 3 of them.