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A multi‐colour/multi‐affinity marker set to visualize phosphoinositide dynamics in Arabidopsis

Overview of attention for article published in Plant Journal, December 2013
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  • Above-average Attention Score compared to outputs of the same age and source (59th percentile)

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Title
A multi‐colour/multi‐affinity marker set to visualize phosphoinositide dynamics in Arabidopsis
Published in
Plant Journal, December 2013
DOI 10.1111/tpj.12358
Pubmed ID
Authors

Mathilde Laetitia Audrey Simon, Matthieu Pierre Platre, Sonia Assil, Ringo van Wijk, William Yawei Chen, Joanne Chory, Marlène Dreux, Teun Munnik, Yvon Jaillais

Abstract

Phosphatidylinositolphosphates (PIPs) are phospholipids that contain a phosphorylated inositol head group. PIPs represent a minor fraction of total phospholipids, but are involved in many regulatory processes, such as cell signalling and intracellular trafficking. Membrane compartments are enriched or depleted in specific PIPs, providing a unique composition for these compartments and contributing to their identity. The precise subcellular localization and dynamics of most PIP species is not fully understood in plants. Here, we designed genetically encoded biosensors with distinct relative affinities and expressed them stably in Arabidopsis thaliana. Analysis of this multi-affinity 'PIPline' marker set revealed previously unrecognized localization of various PIPs in root epidermis. Notably, we found that PI(4,5)P2 is able to localize PIP2 -interacting protein domains to the plasma membrane in non-stressed root epidermal cells. Our analysis further revealed that there is a gradient of PI4P, with the highest concentration at the plasma membrane, intermediate concentration in post-Golgi/endosomal compartments, and the lowest concentration in the Golgi. Finally, we also found a similar gradient of PI3P from high in late endosomes to low in the tonoplast. Our library extends the range of available PIP biosensors, and will allow rapid progress in our understanding of PIP dynamics in plants.

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The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 250 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Czechia 2 <1%
France 2 <1%
Italy 1 <1%
Australia 1 <1%
Unknown 244 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 64 26%
Researcher 56 22%
Student > Master 22 9%
Student > Bachelor 19 8%
Professor > Associate Professor 15 6%
Other 30 12%
Unknown 44 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 119 48%
Biochemistry, Genetics and Molecular Biology 70 28%
Chemistry 5 2%
Medicine and Dentistry 4 2%
Unspecified 2 <1%
Other 4 2%
Unknown 46 18%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 March 2014.
All research outputs
#14,668,917
of 25,477,125 outputs
Outputs from Plant Journal
#5,028
of 7,310 outputs
Outputs of similar age
#173,118
of 320,380 outputs
Outputs of similar age from Plant Journal
#31
of 76 outputs
Altmetric has tracked 25,477,125 research outputs across all sources so far. This one is in the 41st percentile – i.e., 41% of other outputs scored the same or lower than it.
So far Altmetric has tracked 7,310 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.5. This one is in the 30th percentile – i.e., 30% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 320,380 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 76 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 59% of its contemporaries.