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Cloning and characterization of Na+/H+ antiporter (LfNHX1) gene from a halophyte grass Leptochloa fusca for drought and salt tolerance

Overview of attention for article published in Molecular Biology Reports, January 2014
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (73rd percentile)
  • Good Attention Score compared to outputs of the same age and source (77th percentile)

Mentioned by

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1 news outlet

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29 Mendeley
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Title
Cloning and characterization of Na+/H+ antiporter (LfNHX1) gene from a halophyte grass Leptochloa fusca for drought and salt tolerance
Published in
Molecular Biology Reports, January 2014
DOI 10.1007/s11033-013-3015-3
Pubmed ID
Authors

Muhammad Rauf, Khurram Shahzad, Rashid Ali, Moddassir Ahmad, Imran Habib, Shahid Mansoor, Gerald A. Berkowitz, Nasir A. Saeed

Abstract

Abiotic stresses such as salinity and drought have adverse effects on plants. In the present study, a Na(+)/H(+) antiporter gene homologue (LfNHX1) has been cloned from a local halophyte grass (Leptochloa fusca). The LfNHX1 cDNA contains an open reading frame of 1,623 bp that encodes a polypeptide chain of 540 amino acid residues. LfNHX1 protein sequence showed high similarity with NHX1 homologs reported from other halophyte plants. Amino acid and nucleotide sequence similarity, protein topology modeling and the presence of conserved functional domains in the LfNHX1 protein sequence classified it as a vacuolar NHX1 homolog. The overexpression of LfNHX1 gene under CaMV35S promoter conferred salt and drought tolerance in tobacco plants. Under drought stress, transgenic plants showed higher relative water contents, photosynthetic rate, stomatal conductance and membrane stability index as compared to wild type plants. More negative value of leaf osmotic potential was also observed in transgenic plants when compared with wild type control plants. Transgenic plants showed better germination and root growth at 2 mg L(-1) Basta herbicide and three levels (100, 200 and 250 mM) of sodium chloride. These results showed that LfNHX1 is a potential candidate gene for enhancing drought and salt tolerance in crops.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 29 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 28%
Researcher 5 17%
Student > Master 3 10%
Student > Postgraduate 2 7%
Student > Bachelor 2 7%
Other 1 3%
Unknown 8 28%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 34%
Biochemistry, Genetics and Molecular Biology 4 14%
Engineering 2 7%
Earth and Planetary Sciences 1 3%
Computer Science 1 3%
Other 0 0%
Unknown 11 38%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 May 2014.
All research outputs
#6,405,394
of 22,755,127 outputs
Outputs from Molecular Biology Reports
#314
of 2,889 outputs
Outputs of similar age
#76,496
of 306,583 outputs
Outputs of similar age from Molecular Biology Reports
#16
of 72 outputs
Altmetric has tracked 22,755,127 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 2,889 research outputs from this source. They receive a mean Attention Score of 2.2. This one has done well, scoring higher than 88% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 306,583 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.
We're also able to compare this research output to 72 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 77% of its contemporaries.