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Protein Delivery to Vacuole Requires SAND Protein-Dependent Rab GTPase Conversion for MVB-Vacuole Fusion

Overview of attention for article published in Current Biology, May 2014
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Title
Protein Delivery to Vacuole Requires SAND Protein-Dependent Rab GTPase Conversion for MVB-Vacuole Fusion
Published in
Current Biology, May 2014
DOI 10.1016/j.cub.2014.05.005
Pubmed ID
Authors

Manoj K. Singh, Falco Krüger, Hauke Beckmann, Sabine Brumm, Joop E.M. Vermeer, Teun Munnik, Ulrike Mayer, York-Dieter Stierhof, Christopher Grefen, Karin Schumacher, Gerd Jürgens

Abstract

Plasma-membrane proteins such as ligand-binding receptor kinases, ion channels, or nutrient transporters are turned over by targeting to a lytic compartment-lysosome or vacuole-for degradation. After their internalization, these proteins arrive at an early endosome, which then matures into a late endosome with intraluminal vesicles (multivesicular body, MVB) before fusing with the lysosome/vacuole in animals or yeast [1, 2]. The endosomal maturation step involves a SAND family protein mediating Rab5-to-Rab7 GTPase conversion [3]. Vacuolar trafficking is much less well understood in plants [4-6]. Here we analyze the role of the single-copy SAND gene of Arabidopsis. In contrast to its animal or yeast counterpart, Arabidopsis SAND protein is not required for early-to-late endosomal maturation, although its role in mediating Rab5-to-Rab7 conversion is conserved. Instead, Arabidopsis SAND protein is essential for the subsequent fusion of MVBs with the vacuole. The inability of sand mutant to mediate MVB-vacuole fusion is not caused by the continued Rab5 activity but rather reflects the failure to activate Rab7. In conclusion, regarding the endosomal passage of cargo proteins for degradation, a major difference between plants and nonplant organisms might result from the relative timing of endosomal maturation and SAND-dependent Rab GTPase conversion as a prerequisite for the fusion of late endosomes/MVBs with the lysosome/vacuole.

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The data shown below were compiled from readership statistics for 130 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Italy 1 <1%
Australia 1 <1%
Unknown 128 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 30 23%
Researcher 23 18%
Student > Bachelor 13 10%
Student > Master 10 8%
Professor 7 5%
Other 22 17%
Unknown 25 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 67 52%
Biochemistry, Genetics and Molecular Biology 31 24%
Chemistry 2 2%
Medicine and Dentistry 2 2%
Linguistics 1 <1%
Other 2 2%
Unknown 25 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 June 2014.
All research outputs
#22,756,649
of 25,368,786 outputs
Outputs from Current Biology
#14,195
of 14,673 outputs
Outputs of similar age
#207,284
of 240,309 outputs
Outputs of similar age from Current Biology
#174
of 177 outputs
Altmetric has tracked 25,368,786 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
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