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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Gene inactivation using the CRISPR/Cas9 system in the nematode Pristionchus pacificus
|
|---|---|
| Published in |
Development Genes and Evolution, December 2014
|
| DOI | 10.1007/s00427-014-0486-8 |
| Pubmed ID | |
| Authors |
Hanh Witte, Eduardo Moreno, Christian Rödelsperger, Jungeun Kim, Jin-Soo Kim, Adrian Streit, Ralf J. Sommer |
| Abstract |
The diplogastrid nematode Pristionchus pacificus is a nematode model system for comparative studies to Caenorhabditis elegans and integrative evolutionary biology aiming for interdisciplinary approaches of evo-devo, population genetics, and ecology. For this, fieldwork can be combined with laboratory studies, and P. pacificus has a well-developed methodological toolkit of forward genetics, whole genome sequencing, DNA-mediated transformation, and various -omics platforms. Here, we establish CRISPR/Cas9-based gene inactivation and describe various boundary conditions of this methodology for P. pacificus. Specifically, we demonstrate that most mutations arise within the first 9 hours after injections. We systematically tested the efficiency of sgRNAs targeting different exons in Ppa-dpy-1 and characterized the molecular nature of the induced mutations. Finally, we provide a protocol that might also be useful for researchers working with other non-Caenorhabditis nematodes. |
X Demographics
The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 40% |
| Unknown | 3 | 60% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 60% |
| Science communicators (journalists, bloggers, editors) | 2 | 40% |
Mendeley readers
The data shown below were compiled from readership statistics for 87 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 1% |
| Australia | 1 | 1% |
| Unknown | 85 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 23 | 26% |
| Researcher | 13 | 15% |
| Student > Bachelor | 10 | 11% |
| Student > Master | 8 | 9% |
| Student > Doctoral Student | 4 | 5% |
| Other | 9 | 10% |
| Unknown | 20 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 40 | 46% |
| Biochemistry, Genetics and Molecular Biology | 19 | 22% |
| Immunology and Microbiology | 3 | 3% |
| Neuroscience | 3 | 3% |
| Chemistry | 2 | 2% |
| Other | 1 | 1% |
| Unknown | 19 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 April 2015.
All research outputs
#14,599,162
of 23,815,455 outputs
Outputs from Development Genes and Evolution
#334
of 495 outputs
Outputs of similar age
#186,791
of 356,901 outputs
Outputs of similar age from Development Genes and Evolution
#1
of 6 outputs
Altmetric has tracked 23,815,455 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 495 research outputs from this source. They receive a mean Attention Score of 4.3. This one is in the 30th percentile – i.e., 30% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 356,901 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 6 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them