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Mendeley readers
Attention Score in Context
| Title |
Functional expression of an ajmaline pathway-specific esterase from Rauvolfia in a novel plant-virus expression system
|
|---|---|
| Published in |
Planta, August 2005
|
| DOI | 10.1007/s00425-005-0031-0 |
| Pubmed ID | |
| Authors |
Martin Ruppert, Jörn Woll, Anatoli Giritch, Ezzat Genady, Xueyan Ma, Joachim Stöckigt |
| Abstract |
Acetylajmalan esterase (AAE) plays an essential role in the late stage of ajmaline biosynthesis. Based on the partial peptide sequences of AAE isolated and purified from Rauvolfia cell suspensions, a full-length AAE cDNA clone was isolated. The amino acid sequence of AAE has the highest level of identity of 40% to putative lipases known from the Arabidopsis thaliana genome project. Based on the primary structure AAE is a new member of the GDSL lipase superfamily. The expression in Escherichia coli failed although a wide range of conditions were tested. With a novel virus-based plant expression system, it was possible to express AAE functionally in leaves of Nicotiana benthamiana Domin. An extraordinarily high enzyme activity was detected in the Nicotiana tissue, which exceeded that in Rauvolfia serpentina (L.) Benth. ex Kurz cell suspension cultures about 20-fold. This expression allowed molecular analysis of AAE for the first time and increased the number of functionally expressed alkaloid genes from Rauvolfia now to eight, and the number of ajmaline pathway-specific cDNAs to a total of six. |
Mendeley readers
The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 3% |
| Unknown | 37 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 29% |
| Researcher | 8 | 21% |
| Student > Master | 4 | 11% |
| Professor > Associate Professor | 3 | 8% |
| Unspecified | 2 | 5% |
| Other | 5 | 13% |
| Unknown | 5 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 23 | 61% |
| Biochemistry, Genetics and Molecular Biology | 5 | 13% |
| Chemistry | 2 | 5% |
| Nursing and Health Professions | 1 | 3% |
| Unspecified | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 6 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 July 2013.
All research outputs
#7,454,066
of 22,788,370 outputs
Outputs from Planta
#599
of 2,718 outputs
Outputs of similar age
#20,186
of 57,963 outputs
Outputs of similar age from Planta
#9
of 35 outputs
Altmetric has tracked 22,788,370 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,718 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 57,963 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 14th percentile – i.e., 14% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 35 others from the same source and published within six weeks on either side of this one. This one is in the 8th percentile – i.e., 8% of its contemporaries scored the same or lower than it.