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Light sheet-based fluorescence microscopy (LSFM) for the quantitative imaging of cells and tissues

Overview of attention for article published in Cell and Tissue Research, March 2015
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (85th percentile)
  • High Attention Score compared to outputs of the same age and source (94th percentile)

Mentioned by

blogs
1 blog
twitter
2 X users
patent
1 patent

Readers on

mendeley
212 Mendeley
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Title
Light sheet-based fluorescence microscopy (LSFM) for the quantitative imaging of cells and tissues
Published in
Cell and Tissue Research, March 2015
DOI 10.1007/s00441-015-2144-5
Pubmed ID
Authors

Francesco Pampaloni, Bo-Jui Chang, Ernst H. K. Stelzer

Abstract

In light sheet-based fluorescence microscopy (LSFM), only the focal plane is illuminated by a laser light sheet. Hence, only the fluorophores within a thin volume of the specimen are excited. This reduces photo-bleaching and photo-toxic effects by several orders of magnitude compared with any other form of microscopy. Therefore, LSFM (aka single/selective-plane illumination microscopy [SPIM] or digitally scanned light sheet microscopy [DSLM]) is the technique of choice for the three-dimensional imaging of live or fixed and of small or large three-dimensional specimens. The parallel recording of millions of pixels with modern cameras provides an extremely fast acquisition speed. Recent developments address the penetration depth, the resolution and the recording speed of LSFM. The impact of LSFM on research areas such as three-dimensional cell cultures, neurosciences, plant biology and developmental biology is increasing at a rapid pace. The development of high-throughput LSFM is the next leap forward, allowing the application of LSFM in toxicology and drug discovery screening.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 212 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 3 1%
Germany 1 <1%
France 1 <1%
Netherlands 1 <1%
Austria 1 <1%
United States 1 <1%
Unknown 204 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 61 29%
Researcher 42 20%
Student > Master 30 14%
Student > Bachelor 14 7%
Student > Doctoral Student 10 5%
Other 25 12%
Unknown 30 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 55 26%
Engineering 33 16%
Biochemistry, Genetics and Molecular Biology 30 14%
Physics and Astronomy 28 13%
Chemistry 7 3%
Other 22 10%
Unknown 37 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 August 2018.
All research outputs
#2,865,283
of 23,839,820 outputs
Outputs from Cell and Tissue Research
#66
of 2,279 outputs
Outputs of similar age
#36,302
of 260,379 outputs
Outputs of similar age from Cell and Tissue Research
#2
of 36 outputs
Altmetric has tracked 23,839,820 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,279 research outputs from this source. They receive a mean Attention Score of 3.3. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 260,379 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 85% of its contemporaries.
We're also able to compare this research output to 36 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.