| Title |
The genome of cultivated sweet potato contains Agrobacterium T-DNAs with expressed genes: An example of a naturally transgenic food crop
|
|---|---|
| Published in |
Proceedings of the National Academy of Sciences of the United States of America, April 2015
|
| DOI | 10.1073/pnas.1419685112 |
| Pubmed ID | |
| Authors |
Tina Kyndt, Dora Quispe, Hong Zhai, Robert Jarret, Marc Ghislain, Qingchang Liu, Godelieve Gheysen, Jan F. Kreuze |
| Abstract |
Agrobacterium rhizogenes and Agrobacterium tumefaciens are plant pathogenic bacteria capable of transferring DNA fragments [transfer DNA (T-DNA)] bearing functional genes into the host plant genome. This naturally occurring mechanism has been adapted by plant biotechnologists to develop genetically modified crops that today are grown on more than 10% of the world's arable land, although their use can result in considerable controversy. While assembling small interfering RNAs, or siRNAs, of sweet potato plants for metagenomic analysis, sequences homologous to T-DNA sequences from Agrobacterium spp. were discovered. Simple and quantitative PCR, Southern blotting, genome walking, and bacterial artificial chromosome library screening and sequencing unambiguously demonstrated that two different T-DNA regions (IbT-DNA1 and IbT-DNA2) are present in the cultivated sweet potato (Ipomoea batatas [L.] Lam.) genome and that these foreign genes are expressed at detectable levels in different tissues of the sweet potato plant. IbT-DNA1 was found to contain four open reading frames (ORFs) homologous to the tryptophan-2-monooxygenase (iaaM), indole-3-acetamide hydrolase (iaaH), C-protein (C-prot), and agrocinopine synthase (Acs) genes of Agrobacterium spp. IbT-DNA1 was detected in all 291 cultigens examined, but not in close wild relatives. IbT-DNA2 contained at least five ORFs with significant homology to the ORF14, ORF17n, rooting locus (Rol)B/RolC, ORF13, and ORF18/ORF17n genes of A. rhizogenes. IbT-DNA2 was detected in 45 of 217 genotypes that included both cultivated and wild species. Our finding, that sweet potato is naturally transgenic while being a widely and traditionally consumed food crop, could affect the current consumer distrust of the safety of transgenic food crops. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 192 | 15% |
| United Kingdom | 73 | 6% |
| Canada | 45 | 4% |
| Japan | 42 | 3% |
| Spain | 41 | 3% |
| Germany | 36 | 3% |
| France | 27 | 2% |
| Australia | 26 | 2% |
| Peru | 25 | 2% |
| Other | 218 | 17% |
| Unknown | 554 | 43% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 917 | 72% |
| Scientists | 311 | 24% |
| Science communicators (journalists, bloggers, editors) | 27 | 2% |
| Practitioners (doctors, other healthcare professionals) | 23 | 2% |
| Unknown | 1 | <1% |
Mendeley readers
The data shown below were compiled from readership statistics for 832 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 10 | 1% |
| Germany | 7 | <1% |
| Japan | 4 | <1% |
| Italy | 3 | <1% |
| Spain | 3 | <1% |
| Mexico | 3 | <1% |
| Brazil | 3 | <1% |
| France | 2 | <1% |
| United Kingdom | 2 | <1% |
| Other | 15 | 2% |
| Unknown | 780 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 184 | 22% |
| Student > Ph. D. Student | 141 | 17% |
| Student > Bachelor | 112 | 13% |
| Student > Master | 108 | 13% |
| Other | 39 | 5% |
| Other | 152 | 18% |
| Unknown | 96 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 489 | 59% |
| Biochemistry, Genetics and Molecular Biology | 135 | 16% |
| Environmental Science | 12 | 1% |
| Medicine and Dentistry | 10 | 1% |
| Social Sciences | 9 | 1% |
| Other | 51 | 6% |
| Unknown | 126 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1288. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 March 2024.
All research outputs
#10,564
of 25,779,988 outputs
Outputs from Proceedings of the National Academy of Sciences of the United States of America
#336
of 103,729 outputs
Outputs of similar age
#73
of 280,410 outputs
Outputs of similar age from Proceedings of the National Academy of Sciences of the United States of America
#4
of 930 outputs
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