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Complete genome analysis of two new bacteriophages isolated from impetigo strains of Staphylococcus aureus

Overview of attention for article published in Virus Genes, July 2015
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Title
Complete genome analysis of two new bacteriophages isolated from impetigo strains of Staphylococcus aureus
Published in
Virus Genes, July 2015
DOI 10.1007/s11262-015-1223-8
Pubmed ID
Authors

Tibor Botka, Vladislava Růžičková, Hana Konečná, Roman Pantůček, Ivan Rychlík, Zbyněk Zdráhal, Petr Petráš, Jiří Doškař

Abstract

Exfoliative toxin A (ETA)-coding temperate bacteriophages are leading contributors to the toxic phenotype of impetigo strains of Staphylococcus aureus. Two distinct eta gene-positive bacteriophages isolated from S. aureus strains which recently caused massive outbreaks of pemphigus neonatorum in Czech maternity hospitals were characterized. The phages, designated ϕB166 and ϕB236, were able to transfer the eta gene into a prophageless S. aureus strain which afterwards converted into an ETA producer. Complete phage genome sequences were determined, and a comparative analysis of five designed genomic regions revealed major variances between them. They differed in the genome size, number of open reading frames, genome architecture, and virion protein patterns. Their high mutual sequence similarity was detected only in the terminal regions of the genome. When compared with the so far described eta phage genomes, noticeable differences were found. Thus, both phages represent two new lineages of as yet not characterized bacteriophages of the Siphoviridae family having impact on pathogenicity of impetigo strains of S. aureus.

X Demographics

X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Czechia 1 4%
Unknown 25 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 19%
Researcher 5 19%
Student > Master 3 12%
Student > Doctoral Student 2 8%
Student > Bachelor 1 4%
Other 3 12%
Unknown 7 27%
Readers by discipline Count As %
Immunology and Microbiology 4 15%
Agricultural and Biological Sciences 4 15%
Engineering 3 12%
Biochemistry, Genetics and Molecular Biology 3 12%
Medicine and Dentistry 2 8%
Other 2 8%
Unknown 8 31%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 April 2016.
All research outputs
#15,158,251
of 23,498,099 outputs
Outputs from Virus Genes
#496
of 994 outputs
Outputs of similar age
#145,050
of 264,900 outputs
Outputs of similar age from Virus Genes
#6
of 17 outputs
Altmetric has tracked 23,498,099 research outputs across all sources so far. This one is in the 34th percentile – i.e., 34% of other outputs scored the same or lower than it.
So far Altmetric has tracked 994 research outputs from this source. They receive a mean Attention Score of 2.4. This one is in the 49th percentile – i.e., 49% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 264,900 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.